Biomass Conversion and Biorefinery ( IF 3.5 ) Pub Date : 2022-04-20 , DOI: 10.1007/s13399-022-02692-x
Kairat Bekbayev 1 , Akerke Toleugazykyzy 1, 2 , Satenik Mirzoyan 3, 4 , Anait Vassilian 3, 5 , Anna Poladyan 2, 3, 4 , Karen Trchounian 2, 3, 4 , Dinara Tlevlessova 6
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Escherichia coli anaerobically utilize various carbon sources and produce hydrogen (H2) as fermentation end product. Twofold diluted mixture of 10% distiller’s grains (DG), 4% brewer’s spent grains (BSG), and 10% sugar beet molasses (SB) are favorable for enhanced H2 production compared to the single wastes. In wild type cells, H2 production was prolonged till ~ 120 h compared to single waste while in multiple mutant H2 production was determined till ~ 192 h. Specific growth rate was highest in wild type cells in single but not mixed carbon sources reaching to 0.723 h−1 with 20-fold dilution of SB utilization. Cumulative H2 production was ~ 2.7-fold higher in multiple mutant when twofold diluted mixed waste of SB, BSG, and DG was applied compared to wild type reaching to 215 mL. The obtained data can be applied in utilization of agro-industrial waste for production of biomass and bio-H2.
中文翻译:
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甜菜、酒精和啤酒生产废料的单一和混合物利用过程中大肠杆菌的生长和产氢
大肠杆菌厌氧利用各种碳源并产生氢气(H 2)作为发酵终产物。与单一废物相比,10% 酒糟 (DG)、4% 啤酒糟 (BSG) 和 10% 甜菜糖蜜 (SB) 的双重稀释混合物有利于提高 H 2产量。在野生型细胞中,与单一废物相比,H 2的产生延长至~120小时,而在多个突变体中,H 2的产生被确定为~192小时。野生型细胞在单一但非混合碳源中的比生长率最高,达到 0.723 h -1,SB 利用率稀释 20 倍。累积 H 2与达到 215 mL 的野生型相比,当应用 SB、BSG 和 DG 的两倍稀释混合废物时,多个突变体的产量高约 2.7 倍。所得数据可用于农工废弃物生产生物质和bio-H 2的利用。