A quick and selective analytical method was developed via LC–MS/MS for the simultaneous quantitation of alternariol (AOH), alternariol monomethyl ether (AME) and tenuazonic acid (TeA) which belong to the large group of secondary metabolites produced by fungi of the genus Alternaria. Cocoa is susceptible to a number of toxin-producing microorganisms, including Aspergillus and Penicillium species. The method relies on a single-step extraction, followed by an easy clean up, dilution of the raw extract and direct analysis. To assess whether cocoa and chocolate products can be a source of Alternaria toxins, a monitoring of cocoa and chocolate products (N = 99) as well as cocoa raw and semi-finished materials (cocoa shells, cocoa masses; N = 10) was performed. As the results, cocoa and products made from cocoa (without other ingredients) are no source of the Alternaria toxins considered here.

采用 LC-MS/MS 开发了一种快速、选择性的分析方法，用于同时定量由真菌产生的一大类次级代谢产物的交奈酚 (AOH)、交奈酚单甲醚 (AME) 和替萘唑酸 (TeA)。链格孢属。可可对许多产毒微生物敏感，包括曲霉属和青霉属。该方法依赖于一步提取，然后是简单的清理、稀释粗提物和直接分析。为了评估可可和巧克力产品是否可以成为链格孢毒素的来源，对可可和巧克力产品进行监测（N = 99）以及可可原料和半成品（可可壳、可可块；N  = 10）。结果，可可和由可可制成的产品（不含其他成分）不是此处考虑的链格孢毒素的来源。