转录组分析揭示了红光和蓝光诱导的棉花（Gossypium hirsutum L.）中花青素积累的差异。,Frontiers in Plant Science

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转录组分析揭示了红光和蓝光诱导的棉花（Gossypium hirsutum L.）中花青素积累的差异。
Frontiers in Plant Science ( IF 4.1 ) Pub Date : 2022-03-31 , DOI: 10.3389/fpls.2022.788828
Dongnan Shao ₁ , Qian-Hao Zhu ₂ , Qian Liang ₁ , Xuefeng Wang ₁ , Yanjun Li ₁ , Yuqiang Sun ₃ , Xinyu Zhang ₁ , Feng Liu ₁ , Fei Xue ₁ , Jie Sun ₁

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许多因素，包括光照，都会影响植物中花青素的生物合成和积累。光质是影响光诱导花青素生物合成和积累过程的关键因素。我们观察到具有 R1 突变的陆地棉种质汇源的红色在发光二极管（LED）下变为正常的绿色，这启发了我们研究红光和蓝光对花青素生物合成和积累的影响。我们发现红光和蓝光都会增加花青素的积累。比较转录组学分析，包括基因本体论 (GO)、京都基因和基因组百科全书 (KEGG) 和 GSEA，表明在不同光照条件下差异表达的基因富含昼夜节律、苯丙素生物合成、花青素的生物合成，黄酮和黄酮醇的生物合成。毫不奇怪，所有与花青素生物合成相关的主要结构基因，包括关键的调控 MYB 转录因子 (GhPAP1D) 和花青素转运蛋白 (GhGSTF12)，均由红光或蓝光处理诱导。然而，与原花青素生物合成相关的 LARs 和 MATEs 在红光辐射下比蓝光辐射更显着上调。反之亦然，红光下花青素的积累不如蓝光下高。此外，我们证明了 GhHY5（植物昼夜节律的关键调节因子）在调节花青素积累中的潜在作用，这可以通过与 GhPAP1D 的相互作用来实现。一起，这些结果表明红光和蓝光对花色苷生物合成和积累的不同影响，以及包括 GhHY5 和 GhPAP1D 在内的潜在模块在调控棉花花色苷积累中的作用。这些结果还表明，在蓝光下负责合成花青素的底物被转移到红光下原花青素的生物合成。

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Transcriptome Analysis Reveals Differences in Anthocyanin Accumulation in Cotton (Gossypium hirsutum L.) Induced by Red and Blue Light.

Many factors, including illumination, affect anthocyanin biosynthesis and accumulation in plants. light quality is the key factor affecting the process of photoinduced anthocyanin biosynthesis and accumulation. We observed that the red color of the Upland cotton accession Huiyuan with the R1 mutation turned to normal green color under light-emitting diodes (LEDs), which inspired us to investigate the effect of red and blue lights on the biosynthesis and accumulation of anthocyanins. We found that both red and blue lights elevated accumulation of anthocyanins. Comparative transcriptomic analyses, including Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and GSEA, revealed that genes differentially expressed under different light conditions were enriched with the pathways of circadian rhythm, phenylpropanoid biosynthesis, anthocyanin biosynthesis, and flavone and flavonol biosynthesis. Not surprisingly, all the major structural genes related to biosynthesis of anthocyanins, including the key regulatory MYB transcription factor (GhPAP1D) and anthocyanin transporter (GhGSTF12), were induced by red or blue light treatment. However, LARs and MATEs related to biosynthesis of proanthocyanidins were more significantly up-regulated by red light radiation than by blue light radiation. Vice versa, the accumulation of anthocyanins under red light was not as high as that under blue light. In addition, we demonstrated a potential role of GhHY5, a key regulator in plant circadian rhythms, in regulation of anthocyanin accumulation, which could be achieved via interaction with GhPAP1D. Together, these results indicate different effect of red and blue lights on biosynthesis and accumulation of anthocyanins and a potential module including GhHY5 and GhPAP1D in regulation of anthocyanin accumulation in cotton. These results also suggest that the substrates responsible the synthesis of anthocyanins under blue light is diverted to biosynthesis of proanthocyanidin under red light.

更新日期：2022-03-31

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