<br>肠道菌群促进罗非鱼体内砷的生物转化和生物积累,Environmental Pollution

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肠道菌群促进罗非鱼体内砷的生物转化和生物积累
Environmental Pollution ( IF 7.6 ) Pub Date : 2022-04-18 , DOI: 10.1016/j.envpol.2022.119321
Dongdong Song ₁ , Lizhao Chen ₂ , Siqi Zhu ₁ , Li Zhang ₃

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鱼类等水生生物可以积累高水平的砷（As）并将有毒的无机砷（iAs）转化为无毒的砷甜菜碱（AsB）。肠道菌群是否参与鱼类 As 积累和转化过程尚不清楚。在此，我们对罗非鱼（Oreochromis mossambicus）进行抗生素处理 19 天以去除肠道菌群，然后饮食暴露于砷酸盐（As（V）） 16 天。与对照组相比，抗生素处理的鱼在肠道和肌肉中积累的总 As 和 AsB 水平显着降低。对照鱼中的肠道内容物（微生物群、消化液和饮食残渣的混合物）在体外将 As（V）代谢为亚砷酸盐（As（III））和有机砷，而抗生素处理的鱼中的肠道内容物则失去了这种能力。As（V）暴露显着改变了鱼肠道微生物群落。发现嗜麦芽窄食单胞菌是经 As 处理的鱼肠道微生物群中的优势物种（占总操作分类单位（OTU）数量的 >60%）。分离的耐 As 菌株 S. maltophilia SCSIOOM 具有将 As（V）代谢为 As（III）和有机砷酸盐的高能力。总体而言，这些结果表明，肠道微生物群，至少是 As 耐药细菌，参与了鱼类的 As 生物转化途径。

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Gut microbiota promote biotransformation and bioaccumulation of arsenic in tilapia

Aquatic organisms such as fish can accumulate high levels of arsenic (As) and transform toxic inorganic As (iAs) to non-toxic arsenobetaine (AsB). Whether the gut microbiota are involved in the process of As accumulation and transformation in fish is unclear. Herein, we subjected tilapia (Oreochromis mossambicus) to antibiotic treatment for 19 d to remove the gut microbiota, followed by the dietary exposure to arsenate (As(V)) for 16 d. The antibiotic-treated fish accumulated significantly less total As and AsB levels in the intestine and muscle than the fish in the control group. The gut contents (mixture of microbiota, digestive fluid, and diet debris) in the control fish metabolized As(V) to arsenite (As(III)) and organoarsenicals in vitro, while those in the antibiotic-treated fish lost this ability. As(V) exposure significantly changed the fish gut microbiota community. Stenotrophomonas maltophilia was found to be the dominant species (>60% of total operational taxonomic unit (OTU) number) in the gut microbiota of As-treated fish. The isolated As-resistant strain S. maltophilia SCSIOOM owned a high capability to metabolize As(V) to As(III) and organoarsenicals. Overall, these results demonstrated that the gut microbiota, at least the As-resistant bacteria, were involved in As biotransformation pathways in fish.

更新日期：2022-04-18

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