补充双甘氨酸亚铁通过 AMPK/FOXO 途径调节肠道抗氧化能力并重建猪的肠道微生物群和胆汁酸谱,Journal of Agricultural and Food Chemistry

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补充双甘氨酸亚铁通过 AMPK/FOXO 途径调节肠道抗氧化能力并重建猪的肠道微生物群和胆汁酸谱
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2022-04-14 , DOI: 10.1021/acs.jafc.2c00138
Zhenglin Dong ₁ , Dongming Zhang ₁ , Xin Wu ₁ , Yulong Yin ₁ , Dan Wan ₁

Affiliation

本研究采用多组学方法比较硫酸亚铁 (FeSO ₄ ) 和双甘氨酸亚铁 (FebisGly) 在猪体内的风险和益处。FebisGly 组显示血清中甘油三酯 (TG) 和甘油三酯/总胆固醇 (TG/CHOL) 值降低，十二指肠中丙二醛 (MDA) 和谷胱甘肽 (GSH) 水平降低。转录组分析表明，十二指肠中差异表达的基因在 FeSO ₄和 FebisGly 组之间的氧化磷酸化、AMPK 和 FOXO 信号通路中富集。在FebisGly组中AMPK磷酸化和FOXO3蛋白表达显着增加。细菌 16S rRNA 基因序列分析显示 FeSO _{4中的 alpha 多样性显着降低}FebisGly组中厚壁菌门、拟杆菌门和变形菌门的丰度增加。靶向代谢组显示，FebisGly 组中的石胆酸 (LCA)、乙醇石胆酸 (GLCA)、猪去氧胆酸 (HDCA)、熊去氧胆酸 (UDCA) 和糖熊去氧胆酸 (GUDCA) 显着增加。RDA分析表明， FeSO ₄组中梭杆菌与TG和TG/高密度脂蛋白呈正相关，而Christensenellaceae_R-7_group、 Ruminococcaceae_UCG -002和Ruminococcaceae_UCG -005FebisGly 组的 UDCA 和 GLCA 呈正相关。根据目前的研究，FebisGly 可改善血清脂质代谢，通过 AMPK/FOXO 途径调节肠道抗氧化能力，并重建猪的肠道微生物群和胆汁酸谱。

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Ferrous Bisglycinate Supplementation Modulates Intestinal Antioxidant Capacity via the AMPK/FOXO Pathway and Reconstitutes Gut Microbiota and Bile Acid Profiles in Pigs

Multi-omics were applied to compare the risks and benefits of ferrous sulfate (FeSO₄) and ferrous bisglycinate (FebisGly) in pigs in the current study. The FebisGly group showed reduced triglyceride (TG) and triglyceride/total cholesterol (TG/CHOL) values in the serum and reduced malondialdehyde (MDA) and increased glutathione (GSH) levels in the duodenum. Transcriptome analysis revealed that differentially expressed genes in the duodenum were enriched in oxidative phosphorylation, AMPK, and FOXO signaling pathways between FeSO₄ and FebisGly groups. AMPK phosphorylation and FOXO3 protein expressions were significantly increased in the FebisGly group. Bacterial 16S rRNA gene sequence analysis revealed significantly reduced alpha diversity in the FeSO₄ group and increased Firmicutes, reduced Bacteroidetes, and Proteobacteria abundances in the FebisGly group. Targeted metabolome revealed notably increased lithocholic acid (LCA), glycolithocholic acid (GLCA), hyodeoxycholic acid (HDCA), ursodeoxycholic acid (UDCA), and glycoursodeoxycholic acid (GUDCA) in the FebisGly group. RDA analysis indicated that Fusobacteria was positively correlated with TG and TG/high-density lipoprotein in the FeSO₄ group while Christensenellaceae_R-7_group, Ruminococcaceae_UCG-002, and Ruminococcaceae_UCG-005 were positively correlated with UDCA and GLCA in the FebisGly group. According to the current study, FebisGly improves serum lipid metabolism, modulates intestinal antioxidant capacity via the AMPK/FOXO pathway, and reconstitutes gut microbiota and bile acid profiles in pigs.

更新日期：2022-04-14

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