Stable antibacterial cement could contribute to reducing the biofilm formation for a healthy marginal sealing in high-risk areas in indirect restorations. This study aimed to incorporate 1,3,5-triacryloylhexahydro-1,3,5-triazine (TAT) on experimental resin-based cements and evaluate its antibacterial and physicochemical properties. An experimental resin-based cement was formulated with 50 wt% Bisphenol A-Glycidyl Methacrylate (BisGMA), 30 wt% Urethane Dimethacrylate (UDMA), 20 wt% Triethylene Glycol Dimethacrylate (TEGDMA), and initiators. Barium silicate glass was used as a filler (45 wt%) and two different groups were tested: C_TAT with 15 wt% TAT addition and C_CONTROL with no TAT addition. The developed cements were evaluated by their degree of conversion, film thickness, flow, flexural strength, softening solvent, cytotoxicity, and antibacterial activity. Microshear bond strength (μSBS) was evaluated regarding dental tissues, glass-ceramics, and polycrystalline ceramics. C_TAT showed a higher degree of conversion, lower cytotoxicity, lower softening solvent, and an increased film thickness when compared to C_CONTROL. No statistical difference was found between the groups for flow, flexural strength, planktonic antibacterial analysis, and cytotoxicity. Reduced biofilm formation was observed in the C_TAT group. C_TAT improved the μSBS values for a polycrystalline ceramic substrate. The addition of 1,3,5-triacryloylhexahydro-1,3,5-triazine showed anti-biofilm activity, increased degree of conversion, decreased cytotoxicity, and increased bond strength for IPS-YTZP substrate tested.