羧酸酯酶 (CXE) 基因家族的全基因组表达分析表明 GBCXE49 对棉花碱性胁迫的功能反应,BMC Plant Biology

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羧酸酯酶 (CXE) 基因家族的全基因组表达分析表明 GBCXE49 对棉花碱性胁迫的功能反应
BMC Plant Biology ( IF 4.3 ) Pub Date : 2022-04-12 , DOI: 10.1186/s12870-022-03579-9
Cun Rui ₁ , Fanjia Peng ₂ , Yapeng Fan ₁ , Yuexin Zhang ₁ , Zhigang Zhang ₂ , Nan Xu ₁ , Hong Zhang ₁ , Jing Wang ₁ , Shengmei Li ₃ , Tao Yang ₃ , Waqar Afzal Malik ₁ , Xuke Lu ₁ , Xiugui Chen ₁ , Delong Wang ₁ , Chao Chen ₁ , Wenwei Gao ₃ , Wuwei Ye ₁

Affiliation

羧酸酯酶（CXE）是一类广泛存在于动植物和微生物中的具有α/β折叠水解酶活性的水解酶，在植物的生长发育和抗逆性中发挥着重要作用。在Gossypium barbadense、Gossypium hirsutum、Gossypium raimondii和Gossypium arboreum中分别鉴定出72、74、39、38个CXE基因。分析基因结构和表达模式。将GBCXE基因分为6个亚群，绘制了家族成员的染色体分布图。对启动子顺式作用元件的分析表明，大多数GBCXE基因含有与植物激素（GA、IAA）或非生物胁迫相关的顺式元件。我们筛选出的这6个基因在根、茎和叶组织中均有表达。结合热图，选择GBCXE49基因进行亚细胞定位，证实该蛋白在细胞质中表达。该家族4个棉花品种CXE基因的共线性分析表明，串联复制在CXE基因家族的进化中起着不可或缺的作用。GBCXE基因在不同胁迫处理下的表达模式表明GBCXE基因可能通过不同的机制显着参与盐碱胁迫的响应。通过病毒诱导的基因沉默技术（VIGS）推测GBCXE49基因参与了巴巴登对碱胁迫的反应。该家族4个棉花品种CXE基因的共线性分析表明，串联复制在CXE基因家族的进化中起着不可或缺的作用。GBCXE基因在不同胁迫处理下的表达模式表明GBCXE基因可能通过不同的机制显着参与盐碱胁迫的响应。通过病毒诱导的基因沉默技术（VIGS）推测GBCXE49基因参与了巴巴登对碱胁迫的反应。该家族4个棉花品种CXE基因的共线性分析表明，串联复制在CXE基因家族的进化中起着不可或缺的作用。GBCXE基因在不同胁迫处理下的表达模式表明GBCXE基因可能通过不同的机制显着参与盐碱胁迫的响应。通过病毒诱导的基因沉默技术（VIGS）推测GBCXE49基因参与了巴巴登对碱胁迫的反应。

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Genome-wide expression analysis of carboxylesterase (CXE) gene family implies GBCXE49 functional responding to alkaline stress in cotton

Carboxylesterase (CXE) is a type of hydrolase with α/β sheet hydrolase activity widely found in animals, plants and microorganisms, which plays an important role in plant growth, development and resistance to stress. A total of 72, 74, 39, 38 CXE genes were identified in Gossypium barbadense, Gossypium hirsutum, Gossypium raimondii and Gossypium arboreum, respectively. The gene structure and expression pattern were analyzed. The GBCXE genes were divided into 6 subgroups, and the chromosome distribution of members of the family were mapped. Analysis of promoter cis-acting elements showed that most GBCXE genes contain cis-elements related to plant hormones (GA, IAA) or abiotic stress. These 6 genes we screened out were expressed in the root, stem and leaf tissues. Combined with the heat map, GBCXE49 gene was selected for subcellular locate and confirmed that the protein was expressed in the cytoplasm. The collinearity analysis of the CXE genes of the four cotton species in this family indicated that tandem replication played an indispensable role in the evolution of the CXE gene family. The expression patterns of GBCXE gene under different stress treatments indicated that GBCXE gene may significantly participate in the response to salt and alkaline stress through different mechanisms. Through the virus-induced gene silencing technology (VIGS), it was speculated that GBCXE49 gene was involved in the response to alkaline stress in G. barbadense.

更新日期：2022-04-12

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