Glycogen Branching Enzyme with a Novel Chain Transfer Mode Derived from Corallococcus sp. Strain EGB and Its Potential Applications,Journal of Agricultural and Food Chemistry

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Glycogen Branching Enzyme with a Novel Chain Transfer Mode Derived from Corallococcus sp. Strain EGB and Its Potential Applications
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2022-04-11 , DOI: 10.1021/acs.jafc.2c01621
Xianfeng Ye ₁ , Wei Liu ₁ , Yuqi Liao ₁ , Tao Liu ₁ , Yuqiang Zhao _{1,

2} , Yanxin Wang ₁ , Yu Zhang ₁ , Xu Li ₁ , Chengyao Xia ₁ , Xiaodong Fang ₃ , Yan Huang ₁ , Zhoukun Li ₁ , Zhongli Cui ₁ , Fei Wang ₄

Affiliation

Dietary starch with an increased content of resistant starch (RS) has the potential to reduce the prevalence of diabetes, obesity, and cardiovascular diseases. Here, an efficient glycogen branching enzyme, CcGBE, from Corallococcus sp. strain EGB was identified, and its relevant properties, including potential application in the preparation of modified starch, were evaluated. The purified CcGBE exhibited a maximal specific activity of approximately 20,000 U/mg using cassava starch as the optimal substrate. The content of α-1,6-glucosidic bonds in CcGBE-modified cassava starch increased from 2.9 to 13.2%. Meanwhile, both the average chain length (CL) of CcGBE-modified starch and the blue value of the color complex formed by starch and iodine initially increased and then decreased, indicating that a new CL transfer mode was reported. Perforated small starch granules were released after CcGBE treatment, and a time-dependent decrease in the retrogradation enthalpy (ΔH_r) of cassava starch indicated that CcGBE inhibited the long-term retrogradation of starch. Moreover, the RS content and cold water solubility (CWS) of CcGBE-modified starch increased from 3.3 to 12.8% and from 23.1 to 93.8%, respectively. These findings indicate the application potential of CcGBE for the preparation of modified starch with increased RS and CWS.

中文翻译：

源自珊瑚球菌的具有新型链转移模式的糖原支化酶。应变 EGB 及其潜在应用

抗性淀粉 (RS) 含量增加的膳食淀粉有可能降低糖尿病、肥胖症和心血管疾病的患病率。在这里，一种来自珊瑚球菌的高效糖原分支酶CcGBEsp。鉴定了菌株 EGB，并评估了其相关特性，包括在制备改性淀粉中的潜在应用。使用木薯淀粉作为最佳底物，纯化的 CcGBE 表现出约 20,000 U/mg 的最大比活性。CcGBE改性木薯淀粉中α-1,6-糖苷键的含量从2.9%提高到13.2%。同时，CcGBE改性淀粉的平均链长（CL）和淀粉与碘形成的颜色复合物的蓝值均先升高后降低，表明报道了一种新的CL转移模式。CcGBE 处理后释放出穿孔的小淀粉颗粒，并且回生焓随时间降低（Δ H _r) 的木薯淀粉表明 CcGBE 抑制了淀粉的长期回生。此外，CcGBE 改性淀粉的 RS 含量和冷水溶解度 (CWS) 分别从 3.3% 提高到 12.8% 和从 23.1% 提高到 93.8%。这些发现表明 CcGBE 在制备具有增加的 RS 和 CWS 的改性淀粉中的应用潜力。

更新日期：2022-04-11

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