In the present study, a sensitive and simple approach for colorimetric detecting microcystin-LR (MC-LR) in fish tissue was developed based on gold nanoparticles (AuNPs) and a truncated aptamer Apt-910–2, which was obtained by mutation and rational truncation from the previously selected 60-mer one. The 23-mer Apt-910–2 presented improved binding affinity to MC-LR and higher specificity. Under the optimum experimental conditions, the Apt-910–2 colorimetric aptasensor worked well in 10 to 150 ng/mL MC-LR concentration ranges, and the limit of detection (LOD) was 0.38 ng/mL. This Apt-910–2 colorimetric aptasensor was also applied to spiked fish tissue samples and gave recovery rates ranged from 75.18 to 87.72%, with the visual LOD 10 μg/kg in fish tissue judged by the naked eye. Thus, this study provides a rapid, reliable, and convenient approach for visual detection of MC-LR, which is of great significance for food safety control by assessing potential health risks related to seafood consumption.

在本研究中，基于金纳米粒子（AuNPs）和截短适体 Apt-910-2 开发了一种灵敏且简单的比色法检测鱼组织中微囊藻毒素-LR（MC-LR）的方法，该方法是通过突变和理性获得的。从先前选择的 60-mer 截断。23-mer Apt-910-2 对 MC-LR 的结合亲和力和特异性更高。在最佳实验条件下，Apt-910-2 比色适体传感器在 10 至 150 ng/mL MC-LR 浓度范围内工作良好，检测限 (LOD) 为 0.38 ng/mL。该 Apt-910-2 比色适体传感器也应用于加标鱼组织样品，回收率在 75.18% 到 87.72% 之间，肉眼判断鱼组织的视觉 LOD 为 10 μg/kg。因此，本研究提供了一种快速、可靠、