Ascorbic acid can serve as an efficient coreactant for luminol chemiluminescence (CL) reaction. An observable CL emission is produced when mixing ascorbic acid with luminol in an alkaline condition. The CL signal is further dramatically enhanced by cobalt(II) ions. This CL system was exploited as a new method for direct determination of ascorbic acid with a flow injection mode. A good linear response is acquired between the CL signal and ascorbic acid concentration in 5.0–1000.0 ng/mL range with a detection limit of 1.3 ng/mL. The relative standard deviations are 2.3% for intra–day (n = 11) and 3.7% for inter–day (n = 3) by parallel determination of 0.5 μg/mL ascorbic acid solution. The method has good selectivity for ascorbic acid since no obvious responses are observed for a variety of inorganic ions and biological substances. The practicability of the method has been demonstrated by the analysis of ascorbic acid in some soft drinks.

抗坏血酸可作为鲁米诺化学发光 (CL) 反应的有效共反应剂。在碱性条件下将抗坏血酸与鲁米诺混合时会产生可观察到的 CL 发射。钴 (II) 离子进一步显着增强了 CL 信号。该 CL 系统被开发为一种采用流动注射模式直接测定抗坏血酸的新方法。在 5.0–1000.0 ng/mL 范围内的 CL 信号和抗坏血酸浓度之间获得了良好的线性响应，检测限为 1.3 ng/mL。通过平行测定 0.5 μg/mL 抗坏血酸溶液，日内 (n = 11) 的相对标准偏差为 2.3%，日间 (n = 3) 的相对标准偏差为 3.7%。该方法对抗坏血酸具有良好的选择性，对多种无机离子和生物物质均无明显反应。