Near-Infrared Photoacoustic Probe for Reversible Imaging of the ClO–/GSH Redox Cycle In Vivo,Analytical Chemistry

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Near-Infrared Photoacoustic Probe for Reversible Imaging of the ClO–/GSH Redox Cycle In Vivo
Analytical Chemistry ( IF 6.7 ) Pub Date : 2022-04-06 , DOI: 10.1021/acs.analchem.2c00165
Chenming Chan ₁ , Wangning Zhang ₂ , Zhaoli Xue ₁ , Yuanyuan Fang ₁ , Fengxian Qiu ₁ , Jianming Pan ₁ , Jiangwei Tian ₂

Affiliation

Homeostasis of the cellular redox status plays an indispensable role in diverse physiological and pathological processes. Hypochlorite anion (ClO^–) and glutathione (GSH) represent an important redox couple to reflect the redox status in living cells. The current cellular redox probes that detect either ClO^– or GSH alone are not accurate enough to monitor the real redox status. In this work, a reversible photoacoustic (PA) probe, DiOH-BDP, has been synthesized and applied for PA imaging to monitor the ClO^–/GSH couple redox state in an acute liver injury (ALI) model. The near-infrared PA probe DiOH-BDP features significant changes in absorption between 648 and 795 nm during the selective oxidation by ClO^– and the reductive recovery of GSH, which exhibits excellent selectivity and sensitivity toward ClO^– and GSH with the limits of detection of 77.7 nM and 7.2 μM, respectively. Additionally, using PA₇₇₀ as a detection signal allows for the in situ monitoring of the ClO^–/GSH couple, which realizes mapping of the localized redox status of the ALI by the virtue of a PA imaging system. Therefore, the probe provides a potentially technical tool to understand redox imbalance-related pathological formation processes.

中文翻译：

用于体内 ClO–/GSH 氧化还原循环可逆成像的近红外光声探头

细胞氧化还原状态的稳态在多种生理和病理过程中起着不可或缺的作用。次氯酸根阴离子 (ClO ^– ) 和谷胱甘肽 (GSH) 是反映活细胞氧化还原状态的重要氧化还原对。目前仅检测二氧化氯或谷胱甘肽的细胞氧化还原探针^不够准确，无法监测真正的氧化还原状态。在这项工作中，我们合成了一种可逆光声 (PA) 探针DiOH-BDP，并将其应用于 PA 成像，以监测急性肝损伤 (ALI) 模型中的 ClO- ^/ GSH 对氧化还原状态。近红外 PA 探针DiOH-BDP在 ClO 选择性氧化过程中在 648 和 795 nm 之间具有显着的吸收变化^–以及 GSH 的还原回收，它对 ClO 和 GSH 表现出优异的选择性和灵敏度^，检测限分别为 77.7 nM 和 7.2 μM。此外，使用 PA ₇₇₀作为检测信号可以对 ClO-/GSH 对进行原位监测，^通过PA 成像系统实现对 ALI 局部氧化还原状态的映射。因此，该探针提供了一种潜在的技术工具来了解与氧化还原失衡相关的病理形成过程。

更新日期：2022-04-06

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