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Catalytic Hairpin Assembly-Driven Ratiometric Dual-Signal Electrochemical Biosensor for Ultrasensitive Detection of MicroRNA Based on the Ratios of Fe-MOFs and MB-GA-UiO-66-NH2
Analytical Chemistry ( IF 6.7 ) Pub Date : 2022-04-05 , DOI: 10.1021/acs.analchem.1c05293 Jiangbo Dong 1 , Li Wen 1 , Huisi Yang 1 , Jiaying Zhao 1 , Congjuan He 1 , Zhikun Hu 1 , Lan Peng 2 , Changjun Hou 1, 3 , Danqun Huo 1, 4
Analytical Chemistry ( IF 6.7 ) Pub Date : 2022-04-05 , DOI: 10.1021/acs.analchem.1c05293 Jiangbo Dong 1 , Li Wen 1 , Huisi Yang 1 , Jiaying Zhao 1 , Congjuan He 1 , Zhikun Hu 1 , Lan Peng 2 , Changjun Hou 1, 3 , Danqun Huo 1, 4
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In this work, a novel ratio electrochemical biosensing platform based on catalytic hairpin assembly target recovery to trigger dual-signal output was developed for ultrasensitive detection of microRNA (miRNA). To achieve the ratiometric dual-signal strategy, methylene blue (MB), an electrochemical indicator, was ingeniously loaded into the pores of graphene aerogel (GA) and metal–organic framework (MOF) composites with high porosity and large specific surface area, and another electrochemical indicator Fe-MOFs with distinct separation of redox potential was selected as a signal probe. Concretely, with the presence of the target miRNA, the CHA process was initiated and the signal probe was introduced to the electrode surface, producing abundant double-stranded H1-H2@Fe-MOFs-NH2. Then, the measurement and analysis of the prepared ratiometric electrochemical biosensor by differential pulse voltammetry (DPV) showed that the introduction of the target miRNA led to an increase in the oxidation peak signal of Fe-MOFs (+0.8 V) and a decrease in the oxidation peak signal of MB (−0.23 V). Therefore, the peak current ratio of IFe-MOFs/IMB could be employed to accurately reflect the actual concentration of miRNA. Under optimal conditions, the detection limit of the proposed biosensor was down to 50 aM. It was worth noting that the proposed biosensor exhibited excellent detection performance in a complex serum environment and tumor cell lysates, showing great potential in biosensing and clinical diagnosis.
中文翻译：
基于 Fe-MOFs 和 MB-GA-UiO-66-NH2 比例的催化发夹组装驱动比例双信号电化学生物传感器用于超灵敏检测 MicroRNA
在这项工作中，开发了一种基于催化发夹组装目标恢复以触发双信号输出的新型比率电化学生物传感平台，用于超灵敏检测 microRNA (miRNA)。为了实现比例双信号策略，将电化学指示剂亚甲蓝（MB）巧妙地加载到高孔隙率和大比表面积的石墨烯气凝胶（GA）和金属有机骨架（MOF）复合材料的孔隙中，并另一种具有明显分离氧化还原电位的电化学指示剂Fe-MOFs被选为信号探针。具体而言，随着目标miRNA的存在，启动CHA过程并将信号探针引入电极表面，产生丰富的双链H1-H2@Fe-MOFs-NH 2. 然后，通过差分脉冲伏安法 (DPV) 对制备的比率电化学生物传感器进行测量和分析表明，目标 miRNA 的引入导致 Fe-MOFs 的氧化峰信号增加 (+0.8 V)，而在MB的氧化峰信号（-0.23 V）。因此，可以采用I Fe-MOFs / I MB的峰值电流比来准确反映 miRNA 的实际浓度。在最佳条件下，所提出的生物传感器的检测限低至 50 aM。值得注意的是，所提出的生物传感器在复杂的血清环境和肿瘤细胞裂解物中表现出优异的检测性能，在生物传感和临床诊断方面显示出巨大的潜力。
更新日期：2022-04-05
中文翻译：
基于 Fe-MOFs 和 MB-GA-UiO-66-NH2 比例的催化发夹组装驱动比例双信号电化学生物传感器用于超灵敏检测 MicroRNA
在这项工作中，开发了一种基于催化发夹组装目标恢复以触发双信号输出的新型比率电化学生物传感平台，用于超灵敏检测 microRNA (miRNA)。为了实现比例双信号策略，将电化学指示剂亚甲蓝（MB）巧妙地加载到高孔隙率和大比表面积的石墨烯气凝胶（GA）和金属有机骨架（MOF）复合材料的孔隙中，并另一种具有明显分离氧化还原电位的电化学指示剂Fe-MOFs被选为信号探针。具体而言，随着目标miRNA的存在，启动CHA过程并将信号探针引入电极表面，产生丰富的双链H1-H2@Fe-MOFs-NH 2. 然后，通过差分脉冲伏安法 (DPV) 对制备的比率电化学生物传感器进行测量和分析表明，目标 miRNA 的引入导致 Fe-MOFs 的氧化峰信号增加 (+0.8 V)，而在MB的氧化峰信号（-0.23 V）。因此，可以采用I Fe-MOFs / I MB的峰值电流比来准确反映 miRNA 的实际浓度。在最佳条件下，所提出的生物传感器的检测限低至 50 aM。值得注意的是，所提出的生物传感器在复杂的血清环境和肿瘤细胞裂解物中表现出优异的检测性能，在生物传感和临床诊断方面显示出巨大的潜力。
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