Nanocomposite hydrogel composed of Poly N-isopropylacrylamide (PNIPAM), poly N-tert-Butylacrylamide (PBAM) and poly acrylic acid (PAA) was synthesized by free radical polymerization, and then thymol was embedded in it, to design an active antibacterial material that could control release. The characterization of products used SEM, AFM, FTIR, Zeta sizer to analyze the sensitivity of nanoparticles to pH, temperature and salt ions, and the agar diffusion method was used to determine the antibacterial effect of the polymers. The results showed that nanoparticles had pH, temperature and salt ion responsiveness, PNIPAM/PBAM (65:35) nanoparticles loaded thymol had longer release time (more than 24 h) at lower temperature than that (around 6 h) at high temperature. In addition, the nanoparticles could also be adsorbed on the chitosan film, which makes it have a wider range of applications. All thymol-loaded nanoparticles showed antibacterial activity against both B. subtilis and E. coli, while the chitosan film adsorbed nanoparticles showed weak effect, which was related to the controlled and slow release of bacteriostatic agents. Thus, these copolymers have potential value in the development and application of bacteriostatic packaging films for food.