LPS 响应受到城市细颗粒物的影响,International Journal of Molecular Sciences

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LPS 响应受到城市细颗粒物的影响
International Journal of Molecular Sciences ( IF 4.9 ) Pub Date : 2022-04-01 , DOI: 10.3390/ijms23073913
Natália de Souza Xavier Costa ₁ , Gabriel Ribeiro Júnior ₁ , Adair Aparecida Dos Santos Alemany ₁ , Luciano Belotti ₁ , Marcela Frota Cavalcante ₂ , Susan Ribeiro _{3,

4} , Mariana Matera Veras ₁ , Esper Georges Kallás ₃ , Paulo Hilário Nascimento Saldiva ₁ , Marisa Dolhnikoff ₁ , Luiz Fernando Ferraz da Silva ₁

Affiliation

细颗粒物 (PM _2.5 ) 是具有多种化学和物理特性的成分的复杂混合物，与呼吸道和心血管疾病死亡率的增加有关。我们的研究旨在调查暴露于浓缩 PM _2.5对 LPS 诱导的肺损伤发作的影响。BALB/c雄性小鼠暴露于过滤空气或环境细颗粒物 PM _2.5在环境粒子集中器中放置 5 周。然后，用雾化的 LPS 诱导急性肺损伤。在雾化至 LPS 或盐水后 24 小时将动物安乐死。评估了血液、支气管肺泡灌洗液 (BALF) 和肺组织中的炎症细胞和细胞因子（IL-1β、IL-4、IL-5、IL-6、IL-10、IL-17、TNF）。此外，通过立体学方法评估肺形态。我们的研究结果表明，PM+LPS 组显示出损伤的组织学证据，中性粒细胞和巨噬细胞增加的白细胞增多，以及混合的炎症反应谱，其中 KC、IL-6、IL-1β、IL-4 和 IL-17 增加。我们的分析表明，LPS 雾化和 PM _{2.5之间存在相互作用}与单独的 LPS 或 PM _2.5暴露相比，不同地调节炎症反应，具有不同的反应模式。需要进一步的研究来解释 PM _2.5暴露引起的免疫调节机制。

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LPS Response Is Impaired by Urban Fine Particulate Matter

Fine particulate matter (PM_2.5) is a complex mixture of components with diverse chemical and physical characteristics associated with increased respiratory and cardiovascular diseases mortality. Our study aimed to investigate the effects of exposure to concentrated PM_2.5 on LPS-induced lung injury onset. BALB/c male mice were exposed to either filtered air or ambient fine PM_2.5 in an ambient particle concentrator for 5 weeks. Then, an acute lung injury was induced with nebulized LPS. The animals were euthanized 24 h after the nebulization to either LPS or saline. Inflammatory cells and cytokines (IL-1β, IL-4, IL-5, IL-6, IL-10, IL-17, TNF) were assessed in the blood, bronchoalveolar lavage fluid (BALF), and lung tissue. In addition, lung morphology was assessed by stereological methods. Our results showed that the PM+LPS group showed histological evidence of injury, leukocytosis with increased neutrophils and macrophages, and a mixed inflammatory response profile, with increased KC, IL-6, IL-1β, IL-4, and IL-17. Our analysis shows that there is an interaction between the LPS nebulization and PM_2.5 exposure, differently modulating the inflammatory response, with a distinct response pattern as compared to LPS or PM_2.5 exposure alone. Further studies are required to explain the mechanism of immune modulation caused by PM_2.5 exposure.

更新日期：2022-04-01

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