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Promoting Cell Fusion by Polyvalent DNA Ligands
Nano Letters ( IF 9.6 ) Pub Date : 2022-04-01 , DOI: 10.1021/acs.nanolett.2c00216
Fei Gao 1 , Donglei Yang 1 , Fan Xu 1 , Xiaowei Ma 1 , Pengfei Wang 1
Affiliation  

Artificially induced in vitro cell fusion is one essential technique that has been extensively used for biological studies. Nevertheless, there is a lack of robust and efficient method to produce fused cells efficiently. Herein, we proposed to use cell-membrane-anchored polyvalent DNA ligands (PDL) to bring cells into close proximity by forming clusters to enhance PEG-induced cell fusion. PDL of complementary sequences are separately anchored onto different population of cells through cholesterol-induced hydrophobic insertion into lipid membrane. Cells are clustered via mixing cells of complementary PDL prior to cell fusion. PDL exhibited strong stability on cell membrane, induced efficient cell clustering, and eventually achieved cell fusion efficiently in combination with PEG induction. We demonstrated homogeneous and heterogeneous cell fusion of high yield on various cell types. This report presented a programmable yet robust technique for achieving efficient cell fusion that hold great application potentials.

中文翻译:

多价 DNA 配体促进细胞融合

体外人工诱导细胞融合是一项重要的技术,已广泛用于生物学研究。然而,缺乏有效生产融合细胞的稳健和有效的方法。在此,我们建议使用细胞膜锚定的多价 DNA 配体 (PDL) 通过形成簇来使细胞靠近以增强 PEG 诱导的细胞融合。互补序列的 PDL 通过胆固醇诱导的疏水性插入脂质膜分别锚定在不同的细胞群上。在细胞融合之前,通过混合互补 PDL 的细胞来聚集细胞。PDL在细胞膜上表现出很强的稳定性,诱导高效的细胞聚集,最终与PEG诱导相结合,有效地实现细胞融合。我们在各种细胞类型上展示了高产量的同质和异质细胞融合。该报告提出了一种可编程但强大的技术,用于实现具有巨大应用潜力的高效细胞融合。
更新日期:2022-04-01
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