A novel 7-nitro-1,2,3-benzoxadiazole (NBD)-based chemosensor BOP ((5-bromopyridin-2-yl)(4-(7-nitrobenzo[c][1,2,5]oxadiazol-4-yl)piperazin-1-yl)methanone) was synthesized. BOP could detect S²⁻ through fluorescent quenching and colorimetric change. The detection limit was calculated to be 10.9 µM through fluorescence titration. The reaction mechanism of BOP towards S²⁻ was estimated to be thiolysis of NBD amine, producing the cleavage products, NBD-S⁻ and BP ((5-bromopyridin-2-yl)(piperazin-1-yl)methanone). The thiolysis was demonstrated by ¹H NMR titrations, ESI-mass analysis and theoretical calculations. Importantly, BOP was able to successfully monitor S²⁻ in zebrafish and water samples. Additionally, test strips coated with BOP were applied to the in-the-field measurements of S²⁻.

一种基于 7-nitro-1,2,3-benzoxadiazole (NBD) 的新型化学传感器BOP ((5-bromopyridin-2-yl)(4-(7-nitrobenzo[c][1,2,5]oxadiazol-4)合成了-基）哌嗪-1-基）甲酮）。BOP可以通过荧光猝灭和比色变化检测 S ^{2− 。}通过荧光滴定计算检测限为 10.9 µM。BOP对 S ^2-的反应机理估计为 NBD 胺的硫解，产生裂解产物 NBD-S ^-和BP ((5-bromopyridin-2-yl)(piperazin-1-yl)methanone)。通过¹ H NMR 滴定、ESI 质量分析和理论计算证明了硫解作用。重要的，BOP能够成功监测斑马鱼和水样中的 S ²⁻。此外，将涂有BOP的试纸应用于 S ^2-的现场测量。