BAK1 plays contrasting roles in regulating abscisic acid-induced stomatal closure and abscisic acid-inhibited primary root growth in Arabidopsis,Journal of Integrative Plant Biology

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BAK1 plays contrasting roles in regulating abscisic acid-induced stomatal closure and abscisic acid-inhibited primary root growth in Arabidopsis
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2022-03-29 , DOI: 10.1111/jipb.13257
Jinping Deng ₁ , Lingyao Kong ₂ , Yinhua Zhu ₁ , Dan Pei ₁ , Xuexue Chen ₁ , Yu Wang ₁ , Junsheng Qi ₁ , Chunpeng Song ₃ , Shuhua Yang ₁ , Zhizhong Gong _{1,

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Affiliation

The mechanisms that balance plant growth and stress responses are poorly understood, but they appear to involve abscisic acid (ABA) signaling mediated by protein kinases. Here, to explore these mechanisms, we examined the responses of Arabidopsis thaliana protein kinase mutants to ABA treatment. We found that mutants of BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED RECEPTOR KINASE 1 (BAK1) were hypersensitive to the effects of ABA on both seed germination and primary root growth. The kinase OPEN STOMATA 1 (OST1) was more highly activated by ABA in bak1 mutant than the wild type. BAK1 was not activated by ABA treatment in the dominant negative mutant abi1-1 or the pyr1 pyl4 pyl5 pyl8 quadruple mutant, but it was more highly activated by this treatment in the abi1-2 abi2-2 hab1-1 loss-of-function triple mutant than the wild type. BAK1 phosphorylates OST1 T146 and inhibits its activity. Genetic analyses suggested that BAK1 acts at or upstream of core components in the ABA signaling pathway, including PYLs, PP2Cs, and SnRK2s, during seed germination and primary root growth. Although the upstream brassinosteroid (BR) signaling components BAK1 and BR INSENSITIVE 1 (BRI1) positively regulate ABA-induced stomatal closure, mutations affecting downstream components of BR signaling, including BRASSINOSTEROID-SIGNALING KINASEs (BSKs) and BRASSINOSTEROID-INSENSITIVE 2 (BIN2), did not affect ABA-mediated stomatal movement. Thus, our study uncovered an important role of BAK1 in negatively regulating ABA signaling during seed germination and primary root growth, but positively modulating ABA-induced stomatal closure, thus optimizing the plant growth under drought stress.

中文翻译：

BAK1在调节拟南芥脱落酸诱导的气孔关闭和脱落酸抑制的初生根生长中起对比作用

平衡植物生长和应激反应的机制知之甚少，但它们似乎涉及由蛋白激酶介导的脱落酸 (ABA) 信号传导。在这里，为了探索这些机制，我们检查了拟南芥蛋白激酶突变体对 ABA 处理的反应。我们发现油菜甾类不敏感的1-相关受体激酶 1 ( BAK1 ) 的突变体对 ABA 对种子萌发和初生根生长的影响高度敏感。激酶 OPEN STOMATA 1 (OST1) 在bak1突变体中比野生型更能被 ABA 激活。在显性失活突变体abi1-1或pyr1 pyl4 pyl5 pyl8中，BAK1 未被 ABA 处理激活四倍突变体，但这种处理在abi1-2 abi2-2 hab1 - 1中被更高程度地激活功能丧失的三重突变体比野生型。BAK1 磷酸化 OST1 T146 并抑制其活性。遗传分析表明，在种子萌发和初生根生长期间，BAK1 作用于 ABA 信号通路中的核心成分（包括 PYL、PP2C 和 SnRK2）或其上游。尽管上游油菜素内酯 (BR) 信号成分 BAK1 和 BR INSENSITIVE 1 (BRI1) 正向调节 ABA 诱导的气孔关闭，但影响 BR 信号下游成分的突变，包括油菜素类固醇信号激酶 (BSK) 和油菜素类固醇不敏感 2 (BIN2)，不影响 ABA 介导的气孔运动。因此，我们的研究揭示了 BAK1 在种子萌发和初生根生长过程中负调节 ABA 信号传导的重要作用，但正调节 ABA 诱导的气孔关闭，

更新日期：2022-03-29

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