比较三种不同的富含血小板的纤维蛋白膜（L-PRF、A-PRF 和 A-PRF+）的拉伸强度论文：机械和结构体外评估,Polymers

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比较三种不同的富含血小板的纤维蛋白膜（L-PRF、A-PRF 和 A-PRF+）的拉伸强度论文：机械和结构体外评估
Polymers ( IF 4.7 ) Pub Date : 2022-03-29 , DOI: 10.3390/polym14071392
Mara Simões-Pedro ₁ , Pedro Maria B P S Tróia ₁ , Nuno Bernardo Malta Dos Santos ₁ , António M G Completo ₂ , Rogerio Moraes Castilho ₃ , Gustavo Vicentis de Oliveira Fernandes ₃

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应用 PRF（富含血小板的纤维蛋白）衍生膜的预期结果导致缺乏对其一致性和功能完整性的考虑。本研究旨在通过拉伸强度比较机械性能，并分析由 L-PRF（富含白细胞血小板的纤维蛋白）、A-PRF（高级富含血小板纤维蛋白）和 A-PRF+（高级血小板纤维蛋白）产生的膜之间的结构组织-rich fibrin plus)（原始方案），离心速度和时间各不相同。L-PRF (n = 12)、A-PRF (n = 19) 和 A-PRF+ (n = 13) 膜进行了牵引力测试，评估了最大和平均牵引力。对于最大牵引力，0.0020、0.0022 和 0.0010 N·mm ^-2分别获得了 A-PRF、A-PRF+ 和 L-PRF；关于平均牵引阻力，0.0012、0.0015和0.006 N·mm ^-2分别获得 (A-PRF+ > A-PRF > L-PRF)。对于所有研究组，都发现了显着的结果。在通过 SEM 进行的表面形态观察中，L-PRF 基质显示出高度致密的表面，纤维间区域存在粗纤维，红细胞和白细胞明显遭到破坏。A-PRF 协议显示了一个由细长纤维组成的致密基质，这些纤维似乎遵循血小板良好粘附的优先和定向方向。大直径纤维间空间的孔隙率也很明显，而 A-PRF+ 是多孔性最强的血小板浓缩物，具有最大的纤维丰度和细胞保存。因此，本研究得出结论，A-PRF+ 生产的膜具有显着且更高的最大牵引力结果，

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Tensile Strength Essay Comparing Three Different Platelet-Rich Fibrin Membranes (L-PRF, A-PRF, and A-PRF+): A Mechanical and Structural In Vitro Evaluation

Predictable outcomes intended by the application of PRF (platelet-rich fibrin) derivative membranes have created a lack of consideration for their consistency and functional integrity. This study aimed to compare the mechanical properties through tensile strength and analyze the structural organization among the membranes produced by L-PRF (leukocyte platelet-rich fibrin), A-PRF (advanced platelet-rich fibrin), and A-PRF+ (advanced platelet-rich fibrin plus) (original protocols) that varied in centrifugation speed and time. L-PRF (n = 12), A-PRF (n = 19), and A-PRF+ (n = 13) membranes were submitted to a traction test, evaluating the maximum and average traction. For maximum traction, 0.0020, 0.0022, and 0.0010 N·mm⁻² were obtained for A-PRF, A-PRF+, and L-PRF, respectively; regarding the average resistance to traction, 0.0012, 0.0015, and 0.006 N·mm⁻² were obtained, respectively (A-PRF+ > A-PRF > L-PRF). For all groups studied, significant results were found. In the surface morphology observations through SEM, the L-PRF matrix showed a highly compact surface with thick fibers present within interfibrous areas with the apparent destruction of red blood cells and leukocytes. The A-PRF protocol showed a dense matrix composed of thin and elongated fibers that seemed to follow a preferential and orientated direction in which the platelets were well-adhered. Porosity was also evident with a large diameter of the interfibrous spaces whereas A-PRF+ was the most porous platelet concentrate with the greatest fiber abundance and cell preservation. Thus, this study concluded that A-PRF+ produced membranes with significant and higher maximum traction results, indicating a better viscoelastic strength when stretched by two opposing forces.

更新日期：2022-03-29

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