Genetic code expansion in the mouse brain,Nature Chemical Biology

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Genetic code expansion in the mouse brain
Nature Chemical Biology ( IF 12.9 ) Pub Date : 2016-08-29 , DOI: 10.1038/nchembio.2160
Russell J Ernst ₁ , Toke P Krogager ₁ , Elizabeth S Maywood ₁ , Roberto Zanchi ₁ , Václav Beránek ₁ , Thomas S Elliott ₁ , Nicholas P Barry ₁ , Michael H Hastings ₁ , Jason W Chin ₁

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Site-specific incorporation of non-natural amino acids into proteins, via genetic code expansion with pyrrolysyl tRNA synthetase (PylRS) and tRNA^Pyl_CUA pairs (and their evolved derivatives) from Methanosarcina sp., forms the basis of powerful approaches to probe and control protein function in cells and invertebrate organisms. Here we demonstrate that adeno-associated viral delivery of these pairs enables efficient genetic code expansion in primary neuronal culture, organotypic brain slices and the brains of live mice.

中文翻译：

小鼠大脑中的遗传密码扩展

通过使用来自甲烷八叠球菌的吡咯赖氨酰 tRNA 合成酶 (PylRS) 和 tRNA ^Pyl _CUA对（及其进化衍生物）进行遗传密码扩展，将非天然氨基酸定点掺入蛋白质中，形成了强大的探测和控制方法的基础蛋白质在细胞和无脊椎动物中的功能。在这里，我们证明这些对的腺相关病毒传递能够在原代神经元培养物、器官型脑切片和活体小鼠的大脑中有效地扩展遗传密码。

更新日期：2016-08-31

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