Antibacterial protein hydrogels are receiving increasing attention in the aspect of bacteria-infected-wound healing. However, bacterial drug resistance and biofilm infections lead to hard healing of wounds, thus the construction of biological agents that can overcome these issues is essential. Here, a simple and universal method to construct antibiotic-free protein hydrogel with excellent biocompatibility and superior antibacterial activity against drug-resistant bacteria and biofilms was developed. The green industrial microbicide tetrakis (hydroxymethyl) phosphonium sulfate (THPS) as cross-linking agent can be quickly cross-linked with model protein bovine serum albumin (BSA) to form antibacterial hydrogel through simple mixing without any other initiators, subsequently promoting drug-resistance bacteria-infected wound healing. This simple gelatinization strategy allows at least ten different proteins to form hydrogels (e.g. BSA, human serum albumin (HSA), egg albumin, chymotrypsin, trypsin, lysozyme, transferrin, myohemoglobin, hemoglobin, and phycocyanin) under the same conditions, showing prominent universality. Furthermore, drug-resistance bacteria and biofilm could be efficiently destroyed by the representative BSA hydrogel (B-Hydrogel) with antibacterial activity, overcoming biofilm-induced bacterial resistance. The in vivo study demonstrated that the B-Hydrogel as wound dressing can promote reepithelization to accelerate the healing of methicillin-resistant staphylococcus aureus (MRSA)-infected skin wounds without inducing significant side-effect. This readily accessible antibiotic-free protein-based hydrogel not only opens an avenue to provide a facile, feasible and general gelation strategy, but also exhibits promising application in hospital and community MRSA disinfection and treatment.

抗菌蛋白水凝胶在细菌感染伤口愈合方面受到越来越多的关注。然而，细菌耐药性和生物膜感染导致伤口难以愈合，因此构建能够克服这些问题的生物制剂至关重要。这里，开发了一种简单且通用的方法来构建无抗生素的蛋白质水凝胶，该水凝胶具有优异的生物相容性和对耐药细菌和生物膜的卓越抗菌活性。以绿色工业杀菌剂四（羟甲基）硫酸鏻（THPS）为交联剂，无需任何其他引发剂，通过简单混合即可快速与模型蛋白牛血清白蛋白（BSA）交联形成抗菌水凝胶，从而促进耐药性细菌感染的伤口愈合。这种简单的糊化策略允许至少十种不同的蛋白质在相同条件下形成水凝胶（例如BSA、人血清白蛋白（HSA）、卵清蛋白、胰凝乳蛋白酶、胰蛋白酶、溶菌酶、转铁蛋白、肌血红蛋白、血红蛋白和藻蓝蛋白），显示出显着的通用性。此外，具有抗菌活性的代表性BSA水凝胶（B-Hydrogel）可以有效破坏耐药细菌和生物膜，克服生物膜引起的细菌耐药性。体内研究表明，B-Hydrogel作为伤口敷料可以促进上皮再生，加速耐甲氧西林金黄色葡萄球菌（MRSA）感染的皮肤伤口的愈合，且不会引起明显的副作用。 这种易于获得的无抗生素蛋白质水凝胶不仅为提供简便、可行和通用的凝胶策略开辟了道路，而且在医院和社区 MRSA 消毒和治疗方面也展现出了有前景的应用。