1,8-diazafluoren-9-one 聚集体的荧光光谱证据——一种用于指纹痕量检测的前瞻性新超灵敏方法,Journal of Forensic Sciences

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1,8-diazafluoren-9-one 聚集体的荧光光谱证据——一种用于指纹痕量检测的前瞻性新超灵敏方法
Journal of Forensic Sciences ( IF 1.5 ) Pub Date : 2022-03-28 , DOI: 10.1111/1556-4029.15039
Aneta Lewkowicz ₁ , Małgorzata Kantor ₂ , Wojciech Zalewski ₂ , Piotr Bojarski ₁ , Michał Mońka ₁

Affiliation

如果没有分子光谱学，摩擦脊分析就不会成为最受认可的法医学分支之一。每天都使用荧光现象来开发潜在指纹并增强可见的指纹。该研究背后的想法是在各种环境中发现 1,8-diazafluoren-9-one (DFO) 的选定光谱特性。由于其众多优点，这种荧光化合物几十年来一直被常规用于开发潜在指纹，但直到今天，它还没有得到很好的理解。对高染料浓度乙醇中 DFO 的吸收、荧光和激发光谱进行分析，可以识别处于激发态的聚集体。发现染料浓度对荧光光谱和荧光激发光谱有显着影响。特别是，发现染料-主体聚集在极性溶剂中要强得多。从稳态发射光谱可以看出，DFO 聚集体在乙醇中具有强荧光。给出了激发波长对指纹检测效果的影响。已经提出了一种新的反应介质乙醇，与目前使用的甲醇相比，它是无毒的。观察到在该介质中存在处于激发态的 DFO 聚集体，这显着影响指纹痕迹中存在的氨基酸的识别，并在宽光谱范围内发射（绿光）。给出了激发波长对指纹检测效果的影响。已经提出了一种新的反应介质乙醇，与目前使用的甲醇相比，它是无毒的。观察到在该介质中存在处于激发态的 DFO 聚集体，这显着影响指纹痕迹中存在的氨基酸的识别，并在宽光谱范围内发射（绿光）。给出了激发波长对指纹检测效果的影响。已经提出了一种新的反应介质乙醇，与目前使用的甲醇相比，它是无毒的。观察到在该介质中存在处于激发态的 DFO 聚集体，这显着影响指纹痕迹中存在的氨基酸的识别，并在宽光谱范围内发射（绿光）。

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Spectroscopic evidence of fluorescence by 1,8-diazafluoren-9-one aggregates—A prospective new ultrasensitive method for fingerprint trace detection

Friction ridge analysis would not have been one of the most recognized branches of forensics without molecular spectroscopy. The phenomenon of fluorescence is used on daily basis to develop latent fingerprints and to enhance those that are visible. The idea behind the research was to discover selected spectroscopic properties of 1,8-diazafluoren-9-one (DFO) in various environments. This fluorescent compound has been routinely used for decades to develop latent fingerprints due to its numerous advantages, but to this day, it has not been well-understood. Analysis of absorption, fluorescence, and excitation spectra of DFO in ethanol at high dye concentration allowed identification of aggregates in the excited state. A significant influence of the dye concentration on the fluorescence spectra and on the fluorescence excitation spectra was found. In particular, dye-host aggregation was found to be much stronger in a polar solvent. DFO aggregates are strongly fluorescent in ethanol, as can be seen from the steady-state emission spectra. The impact of excitation wavelength on the effect of fingerprint detection is presented. A new reaction medium has been proposed, ethanol, which is nontoxic in relation to the currently used one, methanol. The existence in this medium of DFO aggregates in the excited state, which significantly influences the identification of amino acids present in fingerprint traces, was observed, emitting in a wide spectral range (green light).

更新日期：2022-03-28

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