Sustainable production of chemicals and improving these biosources by engineering metabolic pathways to create efficient plant-based biofactories relies on the knowledge of available chemical/biosynthetic diversity present in the plant. Nicotiana species are well known for their amenability towards transformation and other new plant breeding techniques. The genus Nicotiana is primarily known through Nicotiana tabacum L., the source of tobacco leaves and all respective tobacco products. Due to the prevalence of the latter, N. tabacum and related Nicotiana species are one of the most extensively studied plants. The majority of studies focused solely on N. tabacum or other individual species for chemotyping. The present study analysed a diversity panel including 17 Nicotiana species and six accessions of Nicotiana benthamiana and created a data set that effectively represents the chemotype core collection of the genus Nicotiana. The utilisation of several analytical platforms and previously published libraries/databases enabled the identification and measurement of over 360 metabolites of a wide range of chemical classes as well as thousands of unknowns with dedicated spectral and chromatographic properties.

中文翻译：

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烟草属化学型核心集合

化学品的可持续生产和通过设计代谢途径来改善这些生物资源以创建高效的植物生物工厂依赖于植物中存在的可用化学/生物合成多样性的知识。烟草属物种以其对转化和其他新植物育种技术的顺从性而闻名。烟草属主要通过烟草属烟草L.而闻名，它是烟叶和所有相关烟草产品的来源。由于后者的普遍存在，普通烟草和相关的烟草属物种是研究最广泛的植物之一。大多数研究只关注普通烟草或其他用于化学分型的单个物种。本研究分析了一个多样性面板，包括 17 个烟草属物种和 6 个本氏烟草种质，并创建了一个有效代表烟草属化学型核心集合的数据集。利用多个分析平台和以前发布的库/数据库，能够识别和测量各种化学类别的 360 多种代谢物以及数千种具有专用光谱和色谱特性的未知物。