Separation and identification of collagen peptides derived from enzymatic hydrolysate of Salmo salar skin and their anti-inflammatory activity in lipopolysaccharide (LPS)-induced RAW264.7 inflammatory model,Journal of Food Biochemistry

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Separation and identification of collagen peptides derived from enzymatic hydrolysate of Salmo salar skin and their anti-inflammatory activity in lipopolysaccharide (LPS)-induced RAW264.7 inflammatory model
Journal of Food Biochemistry ( IF 3.5 ) Pub Date : 2022-03-25 , DOI: 10.1111/jfbc.14122
Hui Liu ₁ , Bo Li _{1,

2}

Affiliation

Inflammation is considered as a major risk for the pathogenesis of chronic diseases. Due to the adverse events caused by the long-term use of anti-inflammatory drugs, it is necessary to develop alternative and safe dietary supplements from natural products against inflammation. In this study, flavourzyme hydrolysate (for 0.5 hr) presented the strongest anti-inflammatory activity, which was further separated by ultrafiltration and column chromatography, followed by LC–MS/MS identification. Peptide APD, QA, KA, and WG were identified as anti-inflammatory peptides, which significantly reduced secretion of NO, IL-6, IL-1β, and TNF-α in inflammatory macrophages. Among them, peptide QA showed the best overall anti-inflammatory effect, with the IC₅₀ value against NO production of 849.3 μM. Most of the identified anti-inflammatory peptides were stably against digestion, and they had abundant frequencies in the α (I/II) chain of Salmo salar collagen. Our findings indicated the potential of S. salar skin hydrolysates as functional food to prevent inflammation.

中文翻译：

鲑鱼皮酶解物胶原蛋白肽的分离鉴定及其在脂多糖（LPS）诱导的RAW264.7炎症模型中的抗炎活性

炎症被认为是慢性疾病发病机制的主要风险。由于长期使用抗炎药引起的不良事件，有必要从天然产物中开发替代且安全的抗炎膳食补充剂。在这项研究中，风味酶水解物（0.5 小时）具有最强的抗炎活性，通过超滤和柱层析进一步分离，然后进行 LC-MS/MS 鉴定。肽 APD、QA、KA 和 WG 被鉴定为抗炎肽，可显着减少炎症巨噬细胞中 NO、IL-6、IL-1β 和 TNF-α 的分泌。其中，肽 QA 的整体抗炎效果最好，IC ₅₀对 NO 产生 849.3 μM 的值。大多数已鉴定的抗炎肽对消化具有稳定的作用，并且它们在鲑鱼胶原蛋白的α（I / II）链中具有丰富的频率。我们的研究结果表明S. salar皮肤水解物作为功能性食品预防炎症的潜力。

更新日期：2022-03-25

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