High‐throughput screening highlighted 9‐oxo‐9H‐indeno[1,2‐b]pyrazine‐2,3‐dicarbonitrile (1) as an active inhibitor of ubiquitin‐specific proteases (USPs), a family of hydrolytic enzymes involved in the removal of ubiquitin from protein substrates. The chemical behavior of compound 1 was examined. Moreover, the synthesis and in vitro evaluation of new compounds, analogues of 1, led to the identification of potent and selective inhibitors of the deubiquitinating enzyme USP8.

中文翻译：

---

9-氧-9H-茚并[1,2-b]吡嗪-2,3-二甲腈类似物作为去泛素化酶的潜在抑制剂的合成及生物学评价

高通量筛选突出-9-氧代9 ħ -茚并[1,2 b ]吡嗪-2,3-二腈（1）作为泛素特异性蛋白酶的活性抑制剂（USPS），一个家庭参与水解酶的从蛋白质底物中去除泛素。检查了化合物1的化学行为。此外，新化合物（1的类似物）的合成和体外评估还导致了去泛素化酶USP8的有效和选择性抑制剂的鉴定。