N-乙酰-β-d-己糖胺酶（N-Acetyl-β-d-glucosaminidase）光学传感器研究进展,Bioconjugate Chemistry

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N-乙酰-β-d-己糖胺酶（N-Acetyl-β-d-glucosaminidase）光学传感器研究进展
Bioconjugate Chemistry ( IF 4.0 ) Pub Date : 2022-03-18 , DOI: 10.1021/acs.bioconjchem.2c00057
Janeala J Morsby ₁ , Bradley D Smith ₁

Affiliation

N-乙酰-β- d-己糖胺酶 (EC 3.2.1.52) 是外切作用糖基水解酶，可去除N-乙酰-β- d-葡糖胺 (Glc-NAc) 或N-乙酰-β- d-半乳糖胺 (Gal- NAc) 来自各种生物分子的非还原端，包括低聚糖、糖蛋白和糖脂。相同的酶有时称为N-乙酰基-β- d-氨基葡萄糖苷酶，这篇评论文章使用简写描述符 HEX(NAG) 来表示术语 HEX 或 NAG 在文献中可以互换使用。HEX(NAG) 在整个生物圈的广泛分布及其在溶酶体中的细胞内位置相结合，使其成为食品科学、农业、细胞生物学、医学诊断和化学疗法中的重要酶。50 多年来，研究人员一直使用N -acetyl-β- d的显色衍生物- 用于生物医学研究和临床化学的基础检测中的氨基葡萄糖。最近分子荧光传感器的概念和合成创新，以及仪器的同步技术改进，产生了越来越多的新荧光成像和诊断方法。以下标题提供了 HEX(NAG) 光学传感器最新进展的系统总结：评估肾脏健康、传染病的检测和治疗、癌症的荧光成像、溶酶体疾病的治疗以及化学生物学的反应探针。文章最后对可能的未来方向发表了一些评论。

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Advances in Optical Sensors of N-Acetyl-β-d-hexosaminidase (N-Acetyl-β-d-glucosaminidase)

N-Acetyl-β-d-hexosaminidases (EC 3.2.1.52) are exo-acting glycosyl hydrolases that remove N-acetyl-β-d-glucosamine (Glc-NAc) or N-acetyl-β-d-galactosamine (Gal-NAc) from the nonreducing ends of various biomolecules including oligosaccharides, glycoproteins, and glycolipids. The same enzymes are sometimes called N-acetyl-β-d-glucosaminidases, and this review article employs the shorthand descriptor HEX(NAG) to indicate that the terms HEX or NAG are used interchangeably in the literature. The wide distribution of HEX(NAG) throughout the biosphere and its intracellular location in lysosomes combine to make it an important enzyme in food science, agriculture, cell biology, medical diagnostics, and chemotherapy. For more than 50 years, researchers have employed chromogenic derivatives of N-acetyl-β-d-glucosaminide in basic assays for biomedical research and clinical chemistry. Recent conceptual and synthetic innovations in molecular fluorescence sensors, along with concurrent technical improvements in instrumentation, have produced a growing number of new fluorescent imaging and diagnostics methods. A systematic summary of the recent advances in optical sensors for HEX(NAG) is provided under the following headings: assessing kidney health, detection and treatment of infectious disease, fluorescence imaging of cancer, treatment of lysosomal disorders, and reactive probes for chemical biology. The article concludes with some comments on likely future directions.

更新日期：2022-03-18

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