Background and aims: Vitiligo, a pigmentation-associated disease, affects 1–2% of the global population andis difficult to treat. The pathogenetic mechanism of vitiligo remains unclear. Vernonia anthelmintica (L.) Willd. extract for vitiligo treatment was initially recorded 300 years ago. Itschalcone compounds are believed to play essential roles in this treatment. In a previous study, chalcones were shown to enhance melanin production and tyrosinase activity inmouse B16 cells. Materials and methods: In this study, the effects were investigated of 4-dimethylamino-4'-methoxy chalcone (DMC) on theexpression of tyrosinase (TYR), tyrosinase-related protein (TRP)-1, tyrosinase-relatedprotein (TRP)-2, and microphthalmia-associated transcription factor (MITF)on murine B16 cells. Moreover, the signaling pathways of melanogenesis regulation,and the effects of DMCon the AC/cAMP/PKA/CREB (CREB and p-CREB), p38mitogen-activated protein kinase (MAPK) (MAPK, p-p38 MAPK, ERK andp-ERK), Wnt/β-catenin (β-catenin), and SWI/SNF pathways (SOX-10) proteinexpression levels were examined by Western blot. Results: The data showed that DMCcould promote melanin production by upregulating the p-CREB, p-p38,p-ERK and β-catenin proteins.

中文翻译：

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4-二甲氨基-4'-甲氧基查尔酮促进黑色素合成的机制

背景和目的：白癜风是一种与色素沉着相关的疾病，影响全球 1-2% 的人口并且难以治疗。白癜风的发病机制尚不清楚。Vernonia anthelmintica (L.) Willd. 用于治疗白癜风的提取物最初记录于 300 年前。它的查尔酮化合物被认为在这种治疗中起重要作用。在之前的一项研究中，查尔酮被证明可以增强小鼠 B16 细胞中的黑色素生成和酪氨酸酶活性。材料和方法：本研究探讨了4-二甲基氨基-4'-甲氧基查尔酮（DMC）对酪氨酸酶（TYR）、酪氨酸酶相关蛋白（TRP）-1、酪氨酸酶相关蛋白（TRP）-2和小眼炎表达的影响。小鼠 B16 细胞上的相关转录因子 (MITF)。此外，黑色素生成调控的信号通路，以及 DMCon 对 AC/cAMP/PKA/CREB（CREB ​​和 p-CREB）、p38 丝裂原活化蛋白激酶（MAPK）（MAPK、p-p38 MAPK、ERK 和 p-ERK）的影响)、Wnt/β-连环蛋白 (β-连环蛋白) 和 SWI/SNF 通路 (SOX-10) 蛋白表达水平通过蛋白质印迹检测。结果：数据显示DMC可通过上调p-CREB、p-p38、p-ERK和β-catenin蛋白促进黑色素生成。