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Nanozyme enhanced magnetic immunoassay for dual-mode detection of gastrin-17
Analyst ( IF 3.6 ) Pub Date : 2022-03-16 , DOI: 10.1039/d2an00063f Chujun Zheng 1 , Qixia Jiang 2 , Kan Wang 1 , Tangan Li 1 , Wei Zheng 1 , Yuemeng Cheng 1 , Qihong Ning 1 , Daxiang Cui 1
Analyst ( IF 3.6 ) Pub Date : 2022-03-16 , DOI: 10.1039/d2an00063f Chujun Zheng 1 , Qixia Jiang 2 , Kan Wang 1 , Tangan Li 1 , Wei Zheng 1 , Yuemeng Cheng 1 , Qihong Ning 1 , Daxiang Cui 1
Affiliation
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In this study, we developed a novel magnetic lateral flow assay based on iron oxide decorated with platinum probes (Fe3O4@Pt) for dual-mode detection of gastrin-17 (G-17), which is one of the main biomarkers for early gastric cancer diagnosis. The probe material exhibits both magnetic properties and peroxidase activity. The peroxidase activity enhances the intensity of the brownish coloring of the Fe3O4@Pt probes on the test strip, with a limit of detection of 10 pg mL−1 using the naked eye, which is remarkable for colorimetric lateral flow assays. The magnetic property allows the simple separation and enrichment of the sample, and the signal can be read using a magnetic assay reader for quantitative detection. The linear range for G-17 using the magnetic signal was determined as 10 pg mL−1 to 2200 pg mL−1, and the calculated limit of detection was as low as 3.365 pg mL−1, thereby covering the reference range for G-17. Serum samples were used to validate the test strip, which exhibited high sensitivity, high specificity, and consistency with the results obtained by the enzyme-linked immunosorbent assay method. The entire inspection process using this method can produce results within 35 min and it is simple to operate without requiring strict experimental conditions. This dual-mode lateral flow test strip provides a simple, rapid, and quantitative strategy for detecting G-17, and it may also be valuable in other portable diagnostic applications.
中文翻译：
Nanozyme 增强磁免疫法双模式检测胃泌素 17
在这项研究中，我们开发了一种基于氧化铁的新型磁性横向流动检测方法，该检测方法是用铂探针 (Fe 3 O 4 @Pt) 装饰的，用于双模式检测胃泌素 17 (G-17)，它是主要的生物标志物之一用于早期胃癌诊断。探针材料表现出磁性和过氧化物酶活性。过氧化物酶活性增强了试纸上Fe 3 O 4 @Pt 探针的褐色强度，检测限为 10 pg mL -1使用肉眼，这对于比色侧向流动分析来说是显着的。磁性允许样品的简单分离和富集，并且可以使用磁性测定阅读器读取信号以进行定量检测。使用磁信号测定 G-17 的线性范围为 10 pg mL -1至 2200 pg mL -1，计算的检测限低至 3.365 pg mL -1，从而覆盖 G-17 的参考范围。采用血清样本对试纸条进行验证，该试纸条具有高灵敏度、高特异性，与酶联免疫吸附测定法得到的结果一致。该方法整个检测过程可在35 min内出结果，操作简单，不需要严格的实验条件。这种双模式侧向流动测试条为检测 G-17 提供了一种简单、快速和定量的策略，它在其他便携式诊断应用中也可能很有价值。
更新日期：2022-03-16
中文翻译：
Nanozyme 增强磁免疫法双模式检测胃泌素 17
在这项研究中，我们开发了一种基于氧化铁的新型磁性横向流动检测方法，该检测方法是用铂探针 (Fe 3 O 4 @Pt) 装饰的，用于双模式检测胃泌素 17 (G-17)，它是主要的生物标志物之一用于早期胃癌诊断。探针材料表现出磁性和过氧化物酶活性。过氧化物酶活性增强了试纸上Fe 3 O 4 @Pt 探针的褐色强度，检测限为 10 pg mL -1使用肉眼，这对于比色侧向流动分析来说是显着的。磁性允许样品的简单分离和富集，并且可以使用磁性测定阅读器读取信号以进行定量检测。使用磁信号测定 G-17 的线性范围为 10 pg mL -1至 2200 pg mL -1，计算的检测限低至 3.365 pg mL -1，从而覆盖 G-17 的参考范围。采用血清样本对试纸条进行验证，该试纸条具有高灵敏度、高特异性，与酶联免疫吸附测定法得到的结果一致。该方法整个检测过程可在35 min内出结果，操作简单，不需要严格的实验条件。这种双模式侧向流动测试条为检测 G-17 提供了一种简单、快速和定量的策略，它在其他便携式诊断应用中也可能很有价值。
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