Visualizing Proteins in Mammalian Cells by 19F NMR Spectroscopy,Angewandte Chemie International Edition

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Visualizing Proteins in Mammalian Cells by 19F NMR Spectroscopy
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2022-03-12 , DOI: 10.1002/anie.202201097
Wenkai Zhu _{1,

2} , Alex J Guseman ₁ , Fatema Bhinderwala ₁ , Manman Lu _{1,

2} , Xun-Cheng Su _{3,

4} , Angela M Gronenborn _{1,

2}

Affiliation

One-dimensional ¹⁹F spectra of proteins can be easily detected in mammalian cells in contrast to 2D ¹H-¹⁵N HSQC spectra that can be rendered invisible by interactions with cellular components. Importantly, distances can be measured by ¹⁹F PREs for proteins in their cellular milieu, paving the way for studying structure and dynamics while proteins perform their function.

中文翻译：

通过 19F NMR 光谱法可视化哺乳动物细胞中的蛋白质

蛋白质的一维¹⁹ F 光谱可以在哺乳动物细胞中轻松检测到，而 2D ¹ H- ¹⁵ N HSQC 光谱可以通过与细胞成分的相互作用变得不可见。重要的是，距离可以通过¹⁹ F PRE 测量蛋白质在其细胞环境中的距离，从而为研究蛋白质发挥其功能时的结构和动力学铺平了道路。

更新日期：2022-03-12

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