Acute lung injury (ALI) is a life-threatening acute inflammatory disease with high rates of morbidity and mortality worldwide. 4-Allyl-2,6-dimethoxyphenol (methoxyeugenol), a phenylpropanoid from a synthetic source, exhibits strong anti-inflammatory activity, but its effects on the inflammation of ALI have not yet been reported. In our study, the anti-inflammatory effects of methoxyeugenol were investigated on RAW 264.7 cells and a mice model of ALI. Our results showed that methoxyeugenol (7.5 and 30 µM) attenuated the proliferation and gene expression of interleukin (IL)-6 in LPS-stimulated RAW 264.7 cells. In a mice model of ALI induced with LPS, methoxyeugenol exhibited a significant protective effect, based on influx reduction of macrophages and neutrophils into the lungs; reduction in release of the cytokines IL-6, TNF-α, and IL-10; and in reactive oxygen species (ROS) formation. We show that the anti-inflammatory effects of methoxyeugenol are associated with the suppression of the NFκB signaling pathway. Moreover, we demonstrated for the first time that a phenolic compound, from a synthetic source, protects against lung tissue inflammation and promotes a reduction of NET formation. These findings provided evidence for the use of methoxyeugenol as a new strategy to control inflammation in ALI disease.

急性肺损伤 (ALI) 是一种危及生命的急性炎症性疾病，在全球范围内具有很高的发病率和死亡率。4-Allyl-2,6-dimethoxyphenol (methoxyeugenol) 是一种合成来源的苯丙烷，具有很强的抗炎活性，但尚未报道其对 ALI 炎症的影响。在我们的研究中，甲氧基丁香酚的抗炎作用在 RAW 264.7 细胞和 ALI 小鼠模型上进行了研究。我们的结果表明，甲氧基丁香酚（7.5 和 30 µM）减弱了 LPS 刺激的 RAW 264.7 细胞中白细胞介素 (IL)-6 的增殖和基因表达。在 LPS 诱导的 ALI 小鼠模型中，甲氧基丁香酚显示出显着的保护作用，基于减少巨噬细胞和中性粒细胞流入肺部；减少细胞因子 IL-6、TNF-α 和 IL-10 的释放；和活性氧（ROS）的形成。我们表明甲氧基丁香酚的抗炎作用与抑制 NFκB 信号通路有关。此外，我们首次证明，合成来源的酚类化合物可以防止肺组织炎症并促进减少 NET 的形成。这些发现为使用甲氧基丁香酚作为控制 ALI 疾病炎症的新策略提供了证据。