Characterization of the MicroRNA Profile of Ginger Exosome-like Nanoparticles and Their Anti-Inflammatory Effects in Intestinal Caco-2 Cells,Journal of Agricultural and Food Chemistry

当前位置： X-MOL 学术 › J. Agric. Food Chem. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Characterization of the MicroRNA Profile of Ginger Exosome-like Nanoparticles and Their Anti-Inflammatory Effects in Intestinal Caco-2 Cells
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2022-03-09 , DOI: 10.1021/acs.jafc.1c07306
Lifen Yin ₁ , Ling Yan _{1,

2} , Qian Yu ₁ , Ju Wang ₁ , Changhong Liu _{1,

2} , Lei Wang ₁ , Lei Zheng _{1,

2}

Affiliation

Plant-derived exosome-like nanoparticles (PELNs) have been shown to enter mammalian cells for disease treatment. Although abundant miRNAs are contained in ginger exosome-like nanoparticles (GELNs), little is known about their type and function. Herein, we extracted GELNs with desirable particle sizes (156 ± 36 nm) and a negative surface charge (−26.6 ± 5 mV). The miRNA profiles in ginger and GELNs were analyzed using high-throughput sequencing, and the results of the sequencing were validated by real-time quantitative polymerase chain reaction (RT-qPCR). There were 27 miRNAs with higher expression levels in the GELNs, and they were mainly involved in the regulation of inflammatory and cancer-related pathways. Furthermore, GELNs could be specifically internalized by intestine cells via caveolin-mediated endocytosis and micropinocytosis, as well as counteract lipopolysaccharide (LPS)-induced inflammation by downregulating NF-κβ, IL-6, IL-8, and TNF-α expression. Importantly, the positive effects were further proved to be possibly related to the miRNAs enriched in the GELNs. Overall, these results indicated that PELNs could target human digestive organs and play a cross-kingdom physiological regulation role through miRNAs.

中文翻译：

姜外泌体样纳米颗粒的 MicroRNA 图谱表征及其在肠 Caco-2 细胞中的抗炎作用

植物源性外泌体样纳米颗粒（PELN）已被证明可以进入哺乳动物细胞用于疾病治疗。尽管生姜外泌体样纳米粒子 (GELN) 中含有丰富的 miRNA，但对其类型和功能知之甚少。在这里，我们提取了具有理想粒径（156 ± 36 nm）和负表面电荷（−26.6 ± 5 mV）的 GELN。使用高通量测序分析生姜和 GELN 中的 miRNA 图谱，并通过实时定量聚合酶链反应 (RT-qPCR) 验证测序结果。 GELN中有27个表达量较高的miRNA，它们主要参与炎症和癌症相关通路的调节。此外，GELN 可以通过小窝蛋白介导的内吞作用和小胞饮作用被肠细胞特异性内化，并通过下调 NF-κβ、IL-6、IL-8 和 TNF-α 的表达来抵消脂多糖 (LPS) 诱导的炎症。重要的是，进一步证明这种积极作用可能与 GELN 中富集的 miRNA 有关。总的来说，这些结果表明PELN可以靶向人类消化器官，并通过miRNA发挥跨界的生理调节作用。

更新日期：2022-03-09

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南