Trichothecium spp 的宿主范围和对杀菌剂的敏感性的表征。与田间和储藏中的水果腐烂有关,Plant Pathology

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Trichothecium spp 的宿主范围和对杀菌剂的敏感性的表征。与田间和储藏中的水果腐烂有关
Plant Pathology ( IF 2.3 ) Pub Date : 2022-03-02 , DOI: 10.1111/ppa.13550
Yingbin Li _{1,

2,

3} , Yixiang Liu _{2,

3} , Zhiping Zhang _{1,

2,

3} , Kaiyuan Shen _{2,

3} , Jianjun Hao ₄ , Laixin Luo ₁ , Jianqiang Li ₁

Affiliation

在 2017-2019 年期间，我们观察到了 Trichothecium spp。导致果实在田间和贮藏中腐烂。本研究旨在检查来自不同宿主的物种的形态差异，揭示物种的潜在宿主范围，并评估五种杀菌剂的功效。六株Trichothecium spp。从油桃中分离，选择桃和核桃。虽然菌落形态、菌丝生长速度和孢子大小在宿主之间存在差异，但基于内部转录间隔区和 β-微管蛋白基因 5′端部分的系统发育分析表明，所有测试菌株均属于Trichothecium roseum。对于其寄主范围，使用T检测了 23 种水果。玫瑰香菌株 YT-1 作为接种物；梨、苹果、芒果、板栗、甜瓜、无花果、榴莲等10种水果易感T . 玫瑰，最低接种浓度范围为 10 ⁴至 10 ⁵孢子/ml。最有效抑制六种分离菌的杀菌剂是氟啶胺和氟虫腈，EC ₅₀值分别为 0.07-0.1 和 0.01-0.04 μg/ml，其次是苯醚甲环唑（0.81-2.96 μg/ml）、啶酰菌胺（5.43-13.51 μg） /ml) 和嘧菌酯 (9.18–27.25 μg/ml)。使用异硫氰酸烯丙酯 (AITC)对抗T. 玫瑰香YT-1。应用 10 μl/L AITC 可显着抑制疾病症状。这些发现为该领域未来的疾病应急管理和食品保存提供了有用的信息。

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Characterization of the host range and sensitivity to fungicides of Trichothecium spp. associated with fruit rot in the field and in storage

During 2017–2019, we observed Trichothecium spp. causing fruit rot in the field and in storage. This study was conducted to examine morphological differences of the species from different hosts, reveal the species’ potential host range, and evaluate the efficacy of five fungicides. Six strains of Trichothecium spp. isolated from nectarine, peach and walnut were selected. Although the colony morphology, mycelial growth rate and spore size differed among hosts, phylogenetic analysis based on the internal transcribed spacer and part of the 5ʹ end of the β-tubulin gene showed that all tested strains belonged to Trichothecium roseum. For its host range, 23 kinds of fruit were examined using T. roseum strain YT-1 as an inoculum; 10 kinds of fruit, including pear, apple, mango, Chinese chestnut, pepino melon, fig and durian, were susceptible to T. roseum, with minimum inoculation concentrations ranging from 10⁴ to 10⁵ spores/ml. The fungicides that most effectively inhibited the six isolates were fluazinam and fludioxonil, with EC₅₀ values of 0.07–0.1 and 0.01–0.04 μg/ml, respectively, followed by difenoconazole (0.81–2.96 μg/ml), boscalid (5.43–13.51 μg/ml) and azoxystrobin (9.18–27.25 μg/ml). Improvement of the shelf life of nectarines held in plastic trays was explored using allyl isothiocyanate (AITC) against T. roseum YT-1. The application of 10 μl/L AITC significantly suppressed disease symptoms. The findings provide useful information for future disease emergency management in the field and for food preservation.

更新日期：2022-03-02

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