JPC - Journal of Planar Chromatography - Modern TLC ( IF 1.1 ) Pub Date : 2022-03-03 , DOI: 10.1007/s00764-022-00153-9 Vijay Kumar 1 , Vikram Kushwaha 1 , Yashika Gandhi 1 , Sujeet K. Mishra 1 , Vaibhav Charde 2 , Chandrashekhar Jagtap 2 , G. Babu 2 , Arjun Singh 3 , Ravindra Singh 3 , Narayanam Srikanth 3
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Abstract
A new, simple, selective, sensitive, precise, and robust high-performance thin-layer chromatography (HPTLC) method for the simultaneous quantification of 6-gingerol, guggulsterone E, and guggulsterone Z in coded formulation AYUSH SG-5 (for rheumatoid arthritis) was developed and validated. Analysis of 6-gingerol, guggulsterone E, and guggulsterone Z was performed on thin-layer chromatography (TLC) aluminum plates pre-coated with silica gel 60F254 as the stationary phase. Linear ascending development was carried out in CAMAG twin-trough glass chamber saturated with a mobile phase consisting of toluene–acetone (9:1, V/V) at room temperature (25 ± 2 °C). CAMAG TLC scanner was used for spectrophotometric scanning and analysis in absorbance mode at 280 nm. The system was found to give compact spots for 6-gingerol (RF value 0.149 ± 0.022) scanned at 545 nm and guggulsterone E (RF value 0.192 ± 0.025) and guggulsterone Z (RF value 0.249 ± 0.028) scanned at 254 nm. The linear regression analysis data for the calibration plots exhibited a good linear relationship with r2 = 0.9992 ± 0.02 in the concentration range 100–1200 ng spot–1 with respect to the peak area. The method was validated as per the International Council for Harmonisation (ICH) guidelines to examine the validity, precision, robustness, ruggedness, limits of detection and quantification. The quantified and estimated amount of 6-gingerol, guggulsterone E, and guggulsterone Z in AYUSH SG-5 as well as in raw drugs was found to be well within limits (5% of the labeled content). Statistical analysis of the data manifested that the method is reproducible and sensitive for the simultaneous estimation and quantification of 6-gingerol, guggulsterone E, and guggulsterone Z.
Graphical abstract
中文翻译：
一种经过验证的高效薄层色谱法，用于同时定量用于类风湿性关节炎的编码配方 AYUSH SG-5 中的 6-姜酚、guggulsterone E 和 guggulsterone Z
摘要
一种新的、简单、选择性、灵敏、精确和稳健的高效薄层色谱 (HPTLC) 方法，用于同时定量编码配方 AYUSH SG-5（用于类风湿性关节炎）中的 6-姜酚、guggulsterone E 和 guggulsterone Z ) 已开发和验证。在预涂有硅胶 60F 254作为固定相的薄层色谱 (TLC) 铝板上进行 6-姜酚、guggulsterone E 和 guggulsterone Z 的分析。线性上升显影在 CAMAG 双槽玻璃室中进行，流动相由甲苯 - 丙酮 (9:1, V/V ) 组成。) 在室温 (25 ± 2 °C) 下。CAMAG TLC 扫描仪用于在 280 nm 处以吸光度模式进行分光光度扫描和分析。发现该系统在 545 nm 处扫描的 6-姜酚 ( RF值 0.149 ± 0.022) 和在 254 nm 处扫描的 guggulsterone E (RF值0.192 ± 0.025) 和guggulsterone Z (RF值0.249 ± 0.028)产生紧凑点. 校准曲线的线性回归分析数据在 100–1200 ng 点–1浓度范围内表现出良好的线性关系，r 2 = 0.9992 ± 0.02相对于峰面积。该方法已根据国际协调委员会 (ICH) 指南进行验证，以检查有效性、精密度、稳健性、耐用性、检测限和定量。发现 AYUSH SG-5 以及原料药中 6-姜酚、guggulsterone E 和 guggulsterone Z 的定量和估计量完全在限度内（标记含量的 5%）。数据的统计分析表明，该方法对 6-姜酚、guggulsterone E 和 guggulsterone Z 的同时估计和定量具有重现性和敏感性。
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