Journal of Materials Science ( IF 3.5 ) Pub Date : 2022-03-01 , DOI: 10.1007/s10853-022-06966-7 Yong Li 1, 2, 3 , Xifeng Liu 1, 2 , Bipin Gaihre 1, 2 , Linli Li 1, 2 , Asghar Rezaei 1, 2 , Lichun Lu 1, 2 , A. Lee Miller II 2 , Brian Waletzki 2 , Sungjo Park 4 , Andre Terzic 4
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Hydroxyapatite (HA) is a bioceramic material that shares similar crystal and chemical structures with inorganic components of the bone. However, HA lacks osteoinductive activity and has a brittle nature, making it challenging to apply for direct load-bearing bone applications. In this study, we used a wet chemical method to synthesize zinc-doped hydroxyapatite powders with different Zn/(Zn+Ca) molar ratios of 0, 0.025, 0.05, and 0.1. The corresponding Zn-HA was designated as HA, Zn2.5-HA, Zn5-HA, and Zn10-HA. The Zn-HA powders at 30 wt% were used to fabricate poly(propylene fumarate) (PPF)-based nanocomposite scaffolds (HA/PPF, Zn2.5-HA/PPF, Zn5-HA/PPF, and Zn10-HA/PPF). The physical properties of obtained scaffolds were examined by scanning electron microscopy, energy-dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), and atomic force microscopy (AFM). Live/dead cell viability assay showed that these scaffolds were biocompatible and supported excellent adhesion of MC3T3-E1 preosteoblast cells. Additionally, the proliferation of cells was detected at 1, 4, and 7 days on these scaffolds. Alkaline phosphatase (ALP) activity measurement and alizarin red staining showed good osteogenic differentiation and matrix mineralization for MC3T3-E1 cells growing on these scaffolds. Taken together, the results here indicate that Zn5-HA/PPF nanocomposite scaffolds are promising scaffold material for bone tissue engineering.
Graphical abstract
中文翻译：
用于骨组织工程的锌掺杂羟基磷灰石和聚富马酸丙二醇酯纳米复合支架
羟基磷灰石 (HA) 是一种生物陶瓷材料，与骨骼的无机成分具有相似的晶体和化学结构。然而，HA 缺乏骨诱导活性并且具有脆性，使其难以应用于直接承重骨应用。在本研究中，我们使用湿化学方法合成了不同 Zn/(Zn+Ca) 摩尔比分别为 0、0.025、0.05 和 0.1 的锌掺杂羟基磷灰石粉末。相应的 Zn-HA 被指定为 HA、Zn2.5-HA、Zn5-HA 和 Zn10-HA。30 wt% 的 Zn-HA 粉末用于制备基于聚（富马酸丙二醇酯）（PPF）的纳米复合材料支架（HA/PPF、Zn2.5-HA/PPF、Zn5-HA/PPF 和 Zn10-HA/PPF） ）。通过扫描电子显微镜、能量色散 X 射线光谱 (EDS)、透射电子显微镜 (TEM)、和原子力显微镜（AFM）。活/死细胞活力测定表明，这些支架具有生物相容性，并支持 MC3T3-E1 前成骨细胞的出色粘附。此外，在这些支架上的第 1、4 和 7 天检测到细胞的增殖。碱性磷酸酶 (ALP) 活性测量和茜素红染色显示在这些支架上生长的 MC3T3-E1 细胞具有良好的成骨分化和基质矿化。总之，这里的结果表明，Zn5-HA/PPF 纳米复合材料支架是用于骨组织工程的有前途的支架材料。在这些脚手架上呆了 7 天。碱性磷酸酶 (ALP) 活性测量和茜素红染色显示在这些支架上生长的 MC3T3-E1 细胞具有良好的成骨分化和基质矿化。总之，这里的结果表明，Zn5-HA/PPF 纳米复合材料支架是用于骨组织工程的有前途的支架材料。在这些脚手架上呆了 7 天。碱性磷酸酶 (ALP) 活性测量和茜素红染色显示在这些支架上生长的 MC3T3-E1 细胞具有良好的成骨分化和基质矿化。总之，这里的结果表明，Zn5-HA/PPF 纳米复合材料支架是用于骨组织工程的有前途的支架材料。
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