基于 LC-MS 和 GC-MS 的综合代谢组学揭示植物竞争对黑麦代谢组的影响,Journal of Agricultural and Food Chemistry

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基于 LC-MS 和 GC-MS 的综合代谢组学揭示植物竞争对黑麦代谢组的影响
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2022-02-28 , DOI: 10.1021/acs.jafc.1c06306
Hossein Hazrati _{1,

2} , Per Kudsk ₁ , Ling Ding ₃ , Henriette Uthe ₄ , Inge S Fomsgaard ₁

Affiliation

植物与它们的邻居争夺它们可用的有限资源。处于竞争诱导压力下的植物可能会改变它们的代谢组以增加它们的恢复力或增强它们的防御机制。在本研究中，黑麦 ( Secale graine ) 植物与不同密度（每盆 3、12 和 18 株植物）的奥地利豌豆（Pisum sativum subsp. arvense）、毛豌豆（Vicia villosa）和亚历山大三叶草（Trifolium亚历山大L.) 阐明黑麦代谢组响应不同竞争水平的变化。通过液相色谱三重四极杆串联质谱 (LC-QqQ-MS)、液相色谱四极杆飞行时间质谱 (LC-QTOF-MS) 和气相色谱飞行时间质谱 (GC- TOF-MS）在黑麦植物上进行，并使用单变量和多变量统计分析获得的数据。有针对性的分析表明，高水平的竞争会降低黑麦根中苷元苯并恶嗪 (BX) 的浓度并增加糖苷 BX。非靶向代谢组学分析表明，化感作用化合物 4-羟基苯甲酸和尿嘧啶的黑麦根含量对竞争作出反应。d-焦谷氨酸，它是活性氧 (ROS) 的引发剂。我们的研究结果增强了对植物物种对共培养的生化反应的认识。

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Integrated LC–MS and GC–MS-Based Metabolomics Reveal the Effects of Plant Competition on the Rye Metabolome

Plants compete with their neighbors about the limited resources available to them. Plants under induced stress resulting from competition may alter their metabolome to increase their resilience or enhance their defense mechanisms. In the present study, rye (Secale cereale) plants were cocultivated with different densities (3, 12, and 18 plants per pot) of Austrian pea (Pisum sativum subsp. arvense), hairy vetch (Vicia villosa), and Alexandrian clover (Trifolium alexandrinum L.) to elucidate the changes in the rye metabolome in response to the different levels of competition. Global metabolic profiling by liquid chromatography triple quadrupole tandem mass spectrometry (LC-QqQ-MS), liquid chromatography quadrupole time-of-flight mass spectrometry (LC-QTOF-MS), and gas chromatography time-of-flight mass spectrometry (GC-TOF-MS) was performed on rye plants, and the acquired data were analyzed using uni- and multivariate statistics. Targeted analysis showed that a high level of competition reduced the concentration of aglycone benzoxazinoids (BXs) and increased glycoside BXs in rye roots. Untargeted metabolomics analysis indicated an increase in the rye root content of the allelopathic compounds 4-hydroxybenzoic acid and uracil in response to competition. Untargeted analysis of rye shoots revealed that the plant competition increased the d-pyroglutamic acid, which is an elicitor of reactive oxygen species (ROS). Our results have enhanced the knowledge of the biochemical response of plant species to cocultivation.

更新日期：2022-02-28

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