A highly sensitive PNA-microarray system for miRNA122 recognition,Biotechnology Journal

当前位置： X-MOL 学术 › Biotechnol. J. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

A highly sensitive PNA-microarray system for miRNA122 recognition
Biotechnology Journal ( IF 3.2 ) Pub Date : 2022-02-28 , DOI: 10.1002/biot.202100587
Giuseppe Forte ₁ , Giorgio Ventimiglia ₂ , Massimiliano Pesaturo ₃ , Salvatore Petralia ₁

Affiliation

Surface chemistry is a fundamental aspect of the development of the sensitive biosensor based on microarray technology. Here, an advanced PNA-microarray system for the detection of miRNA, composed by a multilayered Si/Al/Agarose component, is described. A straightforward optical signal enhancement is achieved thanks to a combination of the Al film mirror effect and the positive interference for the emission wavelength of the Cy5 fluorescent label tuned by the agarose film. The PNA-microarray was investigated for the detection of miRNA_122, resulting in a sensitivity of about 1.75 μM^–1 and Limit of Detection in the range of 0.043 nM as a function of the capture probe sequence. The contribution, in terms of H-bonds amounts at 298 and 333 K, of the agarose coating to the dsPNA-RNA interactions was demonstrated by Molecular Dynamic simulations. These results pave the way for advanced sensing strategies suitable for the environmental monitoring and the public safety.

中文翻译：

用于 miRNA122 识别的高灵敏度 PNA 微阵列系统

表面化学是开发基于微阵列技术的灵敏生物传感器的一个基本方面。本文介绍了一种用于检测 miRNA 的先进 PNA 微阵列系统，该系统由多层 Si/Al/Agarose 组件组成。由于 Al 薄膜镜面效应和琼脂糖薄膜调谐的 Cy5 荧光标记发射波长的正干涉相结合，实现了直接的光学信号增强。研究了 PNA 微阵列以检测 miRNA_122，其灵敏度约为 1.75 μM ^–1和 0.043 nM 范围内的检测限作为捕获探针序列的函数。分子动力学模拟证明了琼脂糖涂层对 dsPNA-RNA 相互作用的贡献，就 298 和 333 K 的氢键数量而言。这些结果为适用于环境监测和公共安全的先进传感策略铺平了道路。

更新日期：2022-02-28

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南