Glucuronyl C4 dehydrogenation by the radical SAM enzyme BlsE involved in blasticidin S biosynthesis,Chemical Communications

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Glucuronyl C4 dehydrogenation by the radical SAM enzyme BlsE involved in blasticidin S biosynthesis
Chemical Communications ( IF 4.3 ) Pub Date : 2022-02-15 , DOI: 10.1039/d1cc07132g
He Li ₁ , Junfeng Zhao ₂ , Wei Ding ₂ , Qi Zhang ₁

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Here we report functional investigation of the radical S-adenosylmethionine enzyme BlsE by using cytosylglucuronamide (CGM), which is the amide analog of cytosylglucuronic acid (CGA), an intermediate involved in blasticidin S biosynthesis. We showed that, instead of decarboxylation of CGA reported previously, BlsE catalyzes C4′-dehydrogenation of CGM, and the resulting ketone is acted on by an aminotransferase BlsH to install the C4′-amino group, which uses L-Asp as the amino donor.

中文翻译：

参与杀稻瘟素 S 生物合成的自由基 SAM 酶 BlsE 对葡萄糖醛酸 C4 脱氢

在这里，我们报告了自由基 S-腺苷甲硫氨酸酶 BlsE 的功能研究，通过使用胞嘧啶葡萄糖醛酸 (CGM)，它是胞嘧啶葡萄糖醛酸 (CGA) 的酰胺类似物，一种参与杀稻瘟素 S 生物合成的中间体。我们发现，与之前报道的 CGA 脱羧不同，BlsE 催化 CGM 的 C4'-脱氢，并且生成的酮由转氨酶 BlsH 作用以安装 C4'-氨基，其使用L- Asp 作为氨基供体.

更新日期：2022-02-15

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