Hypochlorous acid (HOCl), one of the most important ROS in living organisms, appears to serve an important role in the immune system in vivo. Endoplasmic reticulum (ER), the largest organelle in cells, manages many biological processes connected to vital activities. To better obtain insight into the relationship of ER stress and HOCl level, a ratiometric fluorescent probe RHE, based on rhodamine combined with HBT and ER-targeting group, was designed and synthesized for HOCl detection in the ER. Probe RHE shows a large stokes shift about 155 nm, which is derived to ESIPT principle. In addition, probe RHE exhibited excellent properties such as fast response (<80 s), high sensitivity with a low detection limit (40 nM), high selectivity and anti-interference. Moreover, probe RHE displayed an excellent ER-targeting ability and had been successfully applied for detection of exogenous and endogenous HOCl in HepG2 cells.

次氯酸 (HOCl) 是生物体内最重要的活性氧之一，似乎在体内免疫系统中发挥着重要作用。内质网 (ER) 是细胞中最大的细胞器，它管理着许多与生命活动相关的生物过程。为了更好地了解内质网应激与次氯酸水平的关系，设计并合成了一种基于罗丹明、HBT和内质网靶向基团的比率荧光探针RHE ，用于内质网中次氯酸的检测。探针RHE显示出大约 155 nm 的大斯托克斯位移，这是源自 ESIPT 原理。此外，探头RHE表现出优异的特性，例如快速响应（<80 s）、高灵敏度和低检测限（40 nM）、高选择性和抗干扰。此外，探针RHE显示出优异的ER靶向能力，已成功应用于检测HepG2细胞中的外源性和内源性HOCl。