Photoprotective Mechanism of Fucoxanthin in Ultraviolet B Irradiation-Induced Retinal Müller Cells Based on Lipidomics Analysis,Journal of Agricultural and Food Chemistry

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Photoprotective Mechanism of Fucoxanthin in Ultraviolet B Irradiation-Induced Retinal Müller Cells Based on Lipidomics Analysis
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2022-02-24 , DOI: 10.1021/acs.jafc.1c07980
Yixin Shi ₁ , Jiaying Ren ₁ , Baomin Zhao ₂ , Taihai Zhu ₂ , Hang Qi ₁

Affiliation

Long-term exposure to sunlight and/or blue light causes vision damage to people of all ages. Dietary pigments and polyphenols have been shown to have photoprotective potential for eyes; however, many unknowns regarding the protective mechanism remain. In this study, we used ultraviolet B (UVB) irradiation-induced retinal Müller cells (RMCs) to screen for dietary polyphenols and pigment compounds with effective photoprotective activity. Fucoxanthin (FX) was shown to have the best therapeutic effect, and the mechanism was evaluated via lipidomics analysis. Both intra- and extracellular ROS, mitochondrial depolarization, and DNA damage induced by UVB irradiation were inhibited by FX. Meanwhile, FX modulated the MAPK signaling pathway, which is correlated with apoptosis and inflammation. Our lipidomics data revealed that FX regulated lipid metabolism disorder and protected the membrane structure. These results confirm the effective photoprotective effects of FX, which may lead to new insights into FX-functionalized photoprotective foods.

中文翻译：

基于脂质组学分析的紫外线 B 照射诱导的视网膜 Müller 细胞中岩藻黄素的光保护机制

长期暴露在阳光和/或蓝光下会对所有年龄段的人造成视力损害。膳食色素和多酚已被证明具有保护眼睛的潜力；然而，关于保护机制的许多未知数仍然存在。在这项研究中，我们使用紫外线 B (UVB) 辐射诱导的视网膜 Müller 细胞 (RMC) 来筛选具有有效光保护活性的膳食多酚和色素化合物。岩藻黄质 (FX) 被证明具有最佳的治疗效果，其作用机制通过脂质组学分析。FX 抑制了 UVB 照射诱导的细胞内和细胞外 ROS、线粒体去极化和 DNA 损伤。同时，FX 调节了与细胞凋亡和炎症相关的 MAPK 信号通路。我们的脂质组学数据显示，FX 调节脂质代谢紊乱并保护膜结构。这些结果证实了 FX 的有效光保护作用，这可能会导致对 FX 功能化光保护食品的新见解。

更新日期：2022-02-24

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