Family Cortinariaceae currently includes only one genus, Cortinarius, which is the largest Agaricales genus, with thousands of species worldwide. The species are important ectomycorrhizal fungi and form associations with many vascular plant genera from tropicals to arctic regions. Genus Cortinarius contains a lot of morphological variation, and its complexity has led many taxonomists to specialize in particular on infrageneric groups. The previous attempts to divide Cortinarius have been shown to be unnatural and the phylogenetic studies done to date have not been able to resolve the higher-level classification of the group above section level. Genomic approaches have revolutionized our view on fungal relationships and provide a way to tackle difficult groups. We used both targeted capture sequencing and shallow whole genome sequencing to produce data and to perform phylogenomic analyses of 75 single-copy genes from 19 species. In addition, a wider 5-locus analysis of 245 species, from the Northern and Southern Hemispheres, was also done. Based on our results, a classification of the family Cortinariaceae into ten genera—Cortinarius, Phlegmacium, Thaxterogaster, Calonarius, Aureonarius, Cystinarius, Volvanarius, Hygronarius, Mystinarius, and Austrocortinarius—is proposed. Seven genera, 10 subgenera, and four sections are described as new to science and five subgenera are introduced as new combinations in a new rank. In addition, 41 section names and 514 species names are combined in new genera and four lecto- and epitypes designated. The position of Stephanopus in suborder Agaricineae remains to be studied. Targeted capture sequencing is used for the first time in fungal taxonomy in Basidiomycetes. It provides a cost-efficient way to produce -omics data in species-rich groups. The -omics data was produced from fungarium specimens up to 21 years old, demonstrating the value of museum specimens in the study of the fungal tree of life. This study is the first family revision in Agaricales based on genomics data and hopefully many others will soon follow.

Cortinariaceae科目前仅包括一个属， Cortinarius ，它是最大的伞菌目属，在全世界有数千种。该物种是重要的外生菌根真菌，与从热带到北极地区的许多维管植物属形成联系。 Cortinarius属包含许多形态变异，其复杂性导致许多分类学家特别专注于属下类群。先前划分Cortinarius 的尝试已被证明是不自然的，迄今为止所做的系统发育研究尚未能够解决科级别以上的群体的更高级别分类。基因组方法彻底改变了我们对真菌关系的看法，并提供了一种解决困难群体的方法。我们使用靶向捕获测序和浅层全基因组测序来生成数据并对来自 19 个物种的 75 个单拷贝基因进行系统发育分析。此外，还对来自北半球和南半球的 245 个物种进行了更广泛的 5 基因座分析。根据我们的研究结果，建议将Cortinariaceae科分为十个属：Cortinarius、Phlegmacium、Thaxterogaster、Calonarius、Aureonarius、Cystinarius、Volvanarius、Hygronarius、Mystinarius和Austrocortinarius 。七个属、十个亚属和四个科被描述为科学上的新事物，五个亚属作为新等级中的新组合被引入。此外，41 个科名和 514 个种名被合并为新属，并指定了 4 个选型和表型。 Stephanopus在伞菌亚目中的地位仍有待研究。 靶向捕获测序首次用于担子菌的真菌分类学。它提供了一种经济高效的方法来生成物种丰富的群体中的组学数据。组学数据是从长达 21 年的真菌标本中产生的，证明了博物馆标本在真菌生命树研究中的价值。这项研究是基于基因组学数据对伞菌目进行的首次家族修订，希望许多其他研究很快就会跟进。