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Biological properties of direct grafting by ultraviolet irradiation of vinyl benzyl phosphonic acid onto titanium surfaces
Reactive & Functional Polymers ( IF 4.5 ) Pub Date : 2022-02-18 , DOI: 10.1016/j.reactfunctpolym.2022.105215
Caroline Pereira 1 , Jean-Sébastien Baumann 1 , Vincent Humbot 2 , Céline Falentin-Daudré 1
Affiliation  

Titanium (Ti) and its alloys are the most effective metals for structural implantable device applications. However, Ti-based materials are passively integrated into the bone, resulting in a purely mechanical attachment. Consequently, the loss of osseointegration often leads to implant failure. Therefore, enhancing bone formation surrounding the implant is primordial. In previous investigations conducted in our laboratory, grafting bioactive polymers with sulfonate groups, such as poly(sodium styrene sulfonate) (polyNaSS), was demonstrated to increase the adherence and differentiation of osteoblast cells. In this context, this contribution proposes to functionalize Ti with a phosphonic acid-based polymer, poly(vinyl benzyl phosphonic acid) (poly(VBP)). A two-step UV-initiated grafting polymerization was developed to covalently graft VBP into Ti surfaces. The surfaces were characterized using colorimetry, Fourier-transformed infrared spectra recorded in an attenuated total reflection (FTIR-ATR), X-ray photoelectron spectroscopy (XPS) techniques, and water contact angle (WCA) measurements. The Ti substrates were evaluated for cell viability, spreading, alkaline phosphatase activity, and calcium formation using MC3T3-E1 osteoblast cells. The interaction of Ti grafted samples with osteoblast cells, was significantly improved as well as the cell/surface interaction. Together, these findings demonstrated that poly(VBP) grafted on Ti surfaces improved osteoblasts' early cell adhesion and spreading activities, crucial for osseointegration applications.



中文翻译:

紫外辐照乙烯基苄基膦酸直接接枝钛表面的生物学性能

钛 (Ti) 及其合金是结构可植入设备应用中最有效的金属。然而,钛基材料被动地集成到骨骼中,从而形成纯机械连接。因此,骨整合的丧失通常会导致种植体失败。因此,增强植入物周围的骨形成是最重要的。在我们实验室之前进行的研究中,已证明将具有磺酸盐基团的生物活性聚合物(如聚苯乙烯磺酸钠)(polyNaSS)接枝可增加成骨细胞的粘附和分化。在这种情况下,该贡献建议使用基于膦酸的聚合物聚(乙烯基苄基膦酸)(聚(VBP))对 Ti 进行功能化。开发了一种两步 UV 引发的接枝聚合,以将 VBP 共价接枝到 Ti 表面。使用比色法、衰减全反射 (FTIR-ATR) 中记录的傅里叶变换红外光谱、X 射线光电子能谱 (XPS) 技术和水接触角 (WCA) 测量来表征表面。使用 MC3T3-E1 成骨细胞评估 Ti 底物的细胞活力、扩散、碱性磷酸酶活性和钙形成。Ti 移植样品与成骨细胞的相互作用以及细胞/表面相互作用显着改善。总之,这些发现表明,在 Ti 表面上移植的聚(VBP)改善了成骨细胞的早期细胞粘附和扩散活动,这对于骨整合应用至关重要。使用比色法、衰减全反射 (FTIR-ATR) 中记录的傅里叶变换红外光谱、X 射线光电子能谱 (XPS) 技术和水接触角 (WCA) 测量来表征表面。使用 MC3T3-E1 成骨细胞评估 Ti 底物的细胞活力、扩散、碱性磷酸酶活性和钙形成。Ti 移植样品与成骨细胞的相互作用以及细胞/表面相互作用显着改善。总之,这些发现表明,在 Ti 表面上移植的聚(VBP)改善了成骨细胞的早期细胞粘附和扩散活动,这对于骨整合应用至关重要。使用比色法、衰减全反射 (FTIR-ATR) 中记录的傅里叶变换红外光谱、X 射线光电子能谱 (XPS) 技术和水接触角 (WCA) 测量来表征表面。使用 MC3T3-E1 成骨细胞评估 Ti 底物的细胞活力、扩散、碱性磷酸酶活性和钙形成。Ti 移植样品与成骨细胞的相互作用以及细胞/表面相互作用显着改善。总之,这些发现表明,在 Ti 表面上移植的聚(VBP)改善了成骨细胞的早期细胞粘附和扩散活动,这对于骨整合应用至关重要。和水接触角(WCA)测量。使用 MC3T3-E1 成骨细胞评估 Ti 底物的细胞活力、扩散、碱性磷酸酶活性和钙形成。Ti 移植样品与成骨细胞的相互作用以及细胞/表面相互作用显着改善。总之,这些发现表明,在 Ti 表面上移植的聚(VBP)改善了成骨细胞的早期细胞粘附和扩散活动,这对于骨整合应用至关重要。和水接触角(WCA)测量。使用 MC3T3-E1 成骨细胞评估 Ti 底物的细胞活力、扩散、碱性磷酸酶活性和钙形成。Ti 移植样品与成骨细胞的相互作用以及细胞/表面相互作用显着改善。总之,这些发现表明,在 Ti 表面上移植的聚(VBP)改善了成骨细胞的早期细胞粘附和扩散活动,这对于骨整合应用至关重要。

更新日期:2022-02-18
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