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Nanoencapsulation as a Promising Platform for the Delivery of the Morin-Cu(II) Complex: Antibacterial and Anticancer Potential
ACS Omega ( IF 3.7 ) Pub Date : 2022-02-22 , DOI: 10.1021/acsomega.1c06956 Pooja Ghosh 1 , Sudipta Bag 1 , Sultana Parveen 1 , Elavarasan Subramani 2 , Koel Chaudhury 2 , Swagata Dasgupta 1
ACS Omega ( IF 3.7 ) Pub Date : 2022-02-22 , DOI: 10.1021/acsomega.1c06956 Pooja Ghosh 1 , Sudipta Bag 1 , Sultana Parveen 1 , Elavarasan Subramani 2 , Koel Chaudhury 2 , Swagata Dasgupta 1
Affiliation
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Nanoencapsulation has emerged as a promising approach for the effective delivery of poorly aqueous soluble compounds. The current study focuses on the preparation of human serum albumin (HSA)-based nanoparticles (NPs) and poly lactic-co-glycolic acid (PLGA)-based nanoparticles for effective delivery of the morin-Cu(II) complex. The NPs were analyzed based on different parameters such as particle size, surface charge, morphology, encapsulation efficiency, and in vitro release properties. The average particle sizes were found to be 214 ± 6 nm for Mor-Cu-HSA-NPs and 185 ± 7.5 nm for Mor-Cu-PLGA-NPs. The release of the morin-Cu(II) complex from both the NPs (Mor-Cu-HSA-NPs and Mor-Cu-PLGA-NPs) followed a biphasic behavior, which comprises an early burst release followed by a sustained and controlled release. The resulting NPs also exhibit free radical scavenging activity confirmed by a standard antioxidant assay. The antibacterial activities of the NPs were investigated using a disk diffusion technique, and it was observed that both the NPs showed better antibacterial activity than morin and the morin-Cu(II) complex. The anticancer activities of the prepared NPs were examined on MDA-MB-468 breast cancer cell lines using a cytotoxicity assay, and the mode of cell death was visualized using fluorescence microscopy. Our results revealed that NPs kill the cancer cells with greater efficiency than free morin and the morin-Cu(II) complex. Thus, both HSA-based NPs and PLGA-based NPs can act as promising delivery systems for the morin-Cu(II) complex and can be utilized for further biomedical applications.
中文翻译：
纳米封装作为传递莫林铜 (II) 复合物的有前途的平台：抗菌和抗癌潜力
纳米封装已成为一种有前途的有效递送水溶性差的化合物的方法。目前的研究重点是制备基于人血清白蛋白 (HSA) 的纳米颗粒 (NPs) 和基于聚乳酸-乙醇酸(PLGA) 的纳米颗粒，以有效递送桑黄素-Cu(II) 复合物。基于不同的参数分析纳米颗粒，如粒径、表面电荷、形态、封装效率和体外释放属性。Mor-Cu-HSA-NPs 的平均粒径为 214 ± 6 nm，Mor-Cu-PLGA-NPs 的平均粒径为 185 ± 7.5 nm。两种 NPs（Mor-Cu-HSA-NPs 和 Mor-Cu-PLGA-NPs）中 morin-Cu(II) 复合物的释放遵循双相行为，包括早期爆发释放，然后是持续和受控释放. 由此产生的 NPs 还表现出通过标准抗氧化剂测定证实的自由基清除活性。使用圆盘扩散技术研究了 NPs 的抗菌活性，观察到 NPs 均显示出比 morin 和 morin-Cu(II) 复合物更好的抗菌活性。使用细胞毒性测定法检查制备的 NPs 对 MDA-MB-468 乳腺癌细胞系的抗癌活性，并使用荧光显微镜观察细胞死亡模式。我们的研究结果表明，NPs 杀死癌细胞的效率高于游离的morin 和morin-Cu(II) 复合物。因此，基于 HSA 的 NPs 和基于 PLGA 的 NPs 都可以作为 morin-Cu(II) 复合物的有前途的递送系统，并可用于进一步的生物医学应用。
更新日期：2022-02-22
中文翻译：
纳米封装作为传递莫林铜 (II) 复合物的有前途的平台：抗菌和抗癌潜力
纳米封装已成为一种有前途的有效递送水溶性差的化合物的方法。目前的研究重点是制备基于人血清白蛋白 (HSA) 的纳米颗粒 (NPs) 和基于聚乳酸-乙醇酸(PLGA) 的纳米颗粒，以有效递送桑黄素-Cu(II) 复合物。基于不同的参数分析纳米颗粒，如粒径、表面电荷、形态、封装效率和体外释放属性。Mor-Cu-HSA-NPs 的平均粒径为 214 ± 6 nm，Mor-Cu-PLGA-NPs 的平均粒径为 185 ± 7.5 nm。两种 NPs（Mor-Cu-HSA-NPs 和 Mor-Cu-PLGA-NPs）中 morin-Cu(II) 复合物的释放遵循双相行为，包括早期爆发释放，然后是持续和受控释放. 由此产生的 NPs 还表现出通过标准抗氧化剂测定证实的自由基清除活性。使用圆盘扩散技术研究了 NPs 的抗菌活性，观察到 NPs 均显示出比 morin 和 morin-Cu(II) 复合物更好的抗菌活性。使用细胞毒性测定法检查制备的 NPs 对 MDA-MB-468 乳腺癌细胞系的抗癌活性，并使用荧光显微镜观察细胞死亡模式。我们的研究结果表明，NPs 杀死癌细胞的效率高于游离的morin 和morin-Cu(II) 复合物。因此，基于 HSA 的 NPs 和基于 PLGA 的 NPs 都可以作为 morin-Cu(II) 复合物的有前途的递送系统，并可用于进一步的生物医学应用。
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