Design of Acetohydroxyacid Synthase Herbicide-Resistant Germplasm through MB-QSAR and CRISPR/Cas9-Mediated Base-Editing Approaches,Journal of Agricultural and Food Chemistry

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Design of Acetohydroxyacid Synthase Herbicide-Resistant Germplasm through MB-QSAR and CRISPR/Cas9-Mediated Base-Editing Approaches
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2022-02-22 , DOI: 10.1021/acs.jafc.1c07180
Tao Wei _{1,

2} , Xin Wen ₂ , Congwei Niu ₂ , Sijing An ₂ , Dawei Wang ₂ , Zhen Xi ₂ , Ning Ning Wang ₁

Affiliation

The development of herbicide-resistant germplasm is significant in solving the increasingly severe weed problem in crop fields. In this study, we, for the first time, rationally designed a predictable and effective approach to create herbicide-resistant germplasm by combining mutation-dependent biomacromolecular quantitative structure–activity relationship (MB-QSAR) and CRISPR/Cas9-mediated base-editing strategies. Our results showed that the homozygous P197F-G654D-G655S or P197F-G654N-G655S Arabidopsis plants exhibited high resistance to multiple acetohydroxyacid synthase-inhibiting herbicides, including chlorsulfuron, bispyribac-sodium, and flucarbazone-sodium. Additionally, the plants with the homozygous P197S mutant displayed increased susceptibility to bispyribac-sodium than the wild-type but more resistance to flumetsulam than other mutants. Besides, we found that the herbicide resistance levels of the gene-edited plants have a good correlation with MB-QSAR prediction.

中文翻译：

通过 MB-QSAR 和 CRISPR/Cas9 介导的碱基编辑方法设计抗乙酰羟酸合酶除草剂种质

抗除草剂种质资源的开发对于解决日益严重的农田杂草问题具有重要意义。在这项研究中，我们首次通过结合突变依赖性生物大分子定量构效关系（MB-QSAR）和CRISPR/Cas9介导的碱基编辑策略，合理设计了一种可预测且有效的方法来创建抗除草剂种质。 . 我们的研究结果表明，纯合的 P197F-G654D-G655S 或 P197F-G654N-G655S 拟南芥植物对多种乙酰羟酸合酶抑制除草剂表现出高抗性，包括氯磺隆、双嘧啶钠和氟卡巴宗钠。此外，与野生型相比，具有纯合 P197S 突变体的植物对双嘧磺草胺的敏感性增加，但对氟美舒兰的抗性高于其他突变体。

更新日期：2022-02-22

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