In this work, we report a facile receptor OMB [N′,N”’-(3-((4-oxochroman-3-yl)methylene)pentane-2,4- diylidene)bis(4-methoxybenzohydrazide)] for the simultaneous detection of toxic analytes (Hg²⁺ and Cd²⁺ ions) in environment and biological samples. The receptor OMB exhibits an excellent selectivity and sensitivity which was determined using absorption and emission spectra. The receptor OMB shows rapid detection with lowest LOD (0.62 nM for Hg²⁺ ions and 0.77 nM for Cd²⁺ ions) and LOQ (2.08 nM for Hg²⁺ ions and 2.57 nM for Cd²⁺ ions) values. In addition, the receptor OMB exhibits 1:1 binding stoichiometry towards Hg²⁺ and Cd²⁺ ions with binding constant values of 5.5 × 10⁶ M⁻¹ and 4.6 × 10⁶ M⁻¹. Moreover, the synthesized receptor OMB possess ability to detect these analytes (Hg²⁺ and Cd²⁺ ions) in realistic samples (food and water) which was recognized using photoluminescence spectroscopy technique. In addition, the receptor OMB is also utilized to detect both the analytes in live HeLa cells. Thus, the overall results indicate that the receptor OMB was more suitable to detect the toxic analytes (Hg²⁺ and Cd²⁺ ions) present in the environment.

在这项工作中，我们报道了一种简单的OMB [N',N”'-(3-((4-oxochroman-3-yl)methylene)pentane-2,4- diylidene)bis(4-methoxybenzohydrazide)] 受体同时检测环境和生物样品中的有毒分析物（Hg ²⁺和 Cd ²⁺离子）。受体OMB表现出优异的选择性和灵敏度，这是使用吸收和发射光谱确定的。受体OMB显示出快速检测，具有最低的 LOD（Hg ^{2+离子为 0.62 nM，Cd 2+}离子为 0.77 nM ）和 LOQ（Hg ^{2+离子为 2.08 nM，Cd 2+}离子为 2.57 nM ）值。此外，受体OMB^{对 Hg 2+}和 Cd ²⁺离子表现出 1:1 的结合化学计量，结合常数值为 5.5 × 10 ⁶  M ^-1和 4.6 × 10 ⁶  M ^-1。此外，合成的OMB受体具有检测实际样品（食物和水）中这些分析物（Hg ²⁺和 Cd ^{2+离子）的能力，这些分析物是使用光致发光光谱技术识别的。}此外，受体OMB还用于检测活 HeLa 细胞中的两种分析物。因此，总体结果表明受体OMB更适合检测有毒分析物 (Hg ²⁺和 Cd ²⁺离子）存在于环境中。