An urgent problem in nursing care is the rising threat of cutaneous wound infections caused by harmful bacteria. In this study, we fabricated a series of cellulose acetate-hyaluronic acid (CA/HA) electrospun fibers loaded with berberine (BBR) using the electrospinning method to determine their antimicrobial performance and potential in in vivo skin wound dressing applications. The BBR-loaded CA/HA electrospun fibers (CA/HA/BBR) were analyzed using scanning electron and Fourier transform infrared microscopies; moreover, their mechanical properties were examined. The analyses demonstrated an average fiber diameter of 502 ± 50 nm; the tensile strength, Young's modulus, and break elongation of CA/HA electrospun fibers were approximately 3.23 ± 0.08 MPa, 17.5 ± 0.03 MPa, and 28.4%, respectively, whereas these values for CA/HA/BBR electrospun fibers were 238 ± 39 nm and 2.99 ± 0.05 MPa, 12.3 ± 0.04 MPa, and 47.8%, respectively. Antimicrobial evaluation of the CA/HA/BBR electrospun fibers demonstrated that the dressings made from these fibers exhibited greater antimicrobial efficacy (>95%) against Staphylococcus aureus and Escherichia coli when compared to that made from CA/HA (>80%) electrospun fibers. In vitro experiments showed that BBR loaded CA/HA electrospun fiber scaffolds have highly enhanced cell viability (>99) and proliferation of L929 fibroblastic cells after 7 days of incubation. In addition, in vivo evaluations in rats showed that the as-fabricated CA/HA/BBR bandage decreased wound size; moreover, it had accelerated healing ability (>95%) and collagen development with increasing treatment duration. These results showed that the addition of BBR enhanced the bioactivity of the dressing without damaging its physical characteristics. Thus, nanostructured dressing made of CA/HA/BBR electrospun fibers has excellent potency for tissue repair in nursing care.

中文翻译：

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改善含有小檗碱负载醋酸纤维素/透明质酸电纺纤维的敷料的抗菌能力，用于皮肤伤口愈合。

护理中的一个紧迫问题是有害细菌引起的皮肤伤口感染的威胁日益增加。在这项研究中，我们使用静电纺丝方法制造了一系列负载黄连素 (BBR) 的醋酸纤维素-透明质酸 (CA/HA) 静电纺丝纤维，以确定它们的体内抗菌性能和潜力。皮肤伤口敷料应用。使用扫描电子和傅里叶变换红外显微镜分析负载 BBR 的 CA/HA 电纺纤维 (CA/HA/BBR)；此外，还检查了它们的机械性能。分析表明平均纤维直径为 502 ± 50 nm；CA/HA 电纺纤维的拉伸强度、杨氏模量和断裂伸长率分别约为 3.23 ± 0.08 MPa、17.5 ± 0.03 MPa 和 28.4%，而 CA/HA/BBR 电纺纤维的这些值分别为 238 ± 39 nm分别为 2.99 ± 0.05 MPa、12.3 ± 0.04 MPa 和 47.8%。CA/HA/BBR 电纺纤维的抗菌性评估表明，由这些纤维制成的敷料对金黄色葡萄球菌和大肠杆菌表现出更高的抗菌功效（>95%）与由 CA/HA (>80%) 电纺纤维制成的相比。体外实验表明，负载 BBR 的 CA/HA 电纺纤维支架在孵育 7 天后具有高度增强的细胞活力 (>99) 和 L929 成纤维细胞的增殖。此外，大鼠体内评估表明，制造的 CA/HA/BBR 绷带减少了伤口大小。此外，随着治疗时间的延长，它具有加速愈合能力（> 95％）和胶原蛋白的发育。这些结果表明，添加 BBR 可增强敷料的生物活性，而不会损害其物理特性。因此，由 CA/HA/BBR 电纺纤维制成的纳米结构敷料在护理中具有出色的组织修复功效。