Sucrose laurate (SL) is a promising dual-functional additive due to its emulsification and antibacterial activity. However, the knowledge on the antibacterial action of SL against Bacillus cereus was lacking, and thus it was investigated from multiple targets. The antibacterial results demonstrated that the minimum inhibitory concentration of SL was 0.3125 mg/mL, and the time-killing curve confirmed the strong antibacterial activity of SL. The alkaline phosphatase assay suggested that SL disrupted the cell wall integrity. The flow cytometry and fluorescence spectroscopy analysis indicated that SL damaged the integrity of cell membrane and dissipated the transmembrane potential, resulting in the leakage of intracellular materials, which were further supported by scanning electron microscopy and transmission electron microscopy. iTRAQ-based proteomic analysis indicated that SL down-regulated cell wall-associated hydrolase, inhibited the synthesis of fatty acids, influenced nucleic acid synthesis, disturbed amino acid metabolism, and blocked HMP pathway and TCA cycle. Finally, the promising application of SL was evidenced in milk beverage. This investigation could provide scientific basis for the practical application of SL as a dual-functional food additive.

蔗糖月桂酸酯 (SL) 具有乳化和抗菌活性，是一种很有前途的双功能添加剂。然而，关于 SL 对蜡状芽孢杆菌的抗菌作用的知识缺乏，因此从多个目标进行了调查。抗菌结果表明，SL的最低抑菌浓度为0.3125 mg/mL，时间-杀灭曲线证实了SL的强抑菌活性。碱性磷酸酶测定表明 SL 破坏了细胞壁的完整性。流式细胞仪和荧光光谱分析表明，SL破坏了细胞膜的完整性，耗散了跨膜电位，导致细胞内物质泄漏，扫描电镜和透射电镜进一步证实了这一点。基于 iTRAQ 的蛋白质组学分析表明，SL 下调细胞壁相关水解酶，抑制脂肪酸合成，影响核酸合成，扰乱氨基酸代谢，并阻断 HMP 途径和 TCA 循环。最后，SL 在乳饮料中的应用前景广阔。该研究可为SL作为双功能食品添加剂的实际应用提供科学依据。