无花果 DPPH 自由基清除活性和定量植物化学分析测定抗氧化剂,Molecules

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无花果 DPPH 自由基清除活性和定量植物化学分析测定抗氧化剂
Molecules ( IF 4.2 ) Pub Date : 2022-02-17 , DOI: 10.3390/molecules27041326
Siddartha Baliyan , Riya Mukherjee , Anjali Priyadarshini , Arpana Vibhuti , Archana Gupta , Ramendra Pati Pandey , Chung-Ming Chang

使用 F. religiosa 可能对炎症性疾病有益，并可用于各种健康状况。在本文中，我们研究了利用 (DPPH) 2,2-Diphenyl-1-picrylhydrazylradical 清除榕树中抗氧化剂的活性，因为榕树是一种重要的草本植物，它的每个部分都具有多种药用特性，例如研究人员可用于开发和设计各种新药的抗菌特性。2,2-Diphenyl-1-picrylhydrazyl (DPPH) 是一种流行、快速、简单且经济实惠的抗氧化性能测量方法，包括使用自由基来评估物质作为氢气提供者的潜力或自由基清除剂 (FRS)。DPPH 测试技术与消除 DPPH 相关，DPPH 是一种稳定的自由基。自由基 DPPH 与奇数电子相互作用，在 517 nm 处产生强吸光度，即紫色色调。例如，FRS 抗氧化剂与 DPPH 反应形成 DPPHH，由于氢含量较低，DPPHH 的吸光度低于 DPPH。与 DPPH-H 形式相比，它是激进的，因为随着吸收的电子数量的增加，它会导致脱色或黄色调。脱色显着影响降低能力。一旦 DPPH 溶液与氢原子源结合，就会形成二苯基苦基肼的低态，并褪去紫罗兰色。解释 DPPH 测试背后的过程，以及它们对 Ficus religiosa (F. religiosa）在金属氧化物纳米颗粒（特别是 MgO）的制造中，以及它们对抗氧化剂的影响，从测试中选择了一个样本进行进一步研究。根据我们的发现，F. religiosa 具有抗氧化特性，可用于治疗由自由基引起的疾病。

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Determination of Antioxidants by DPPH Radical Scavenging Activity and Quantitative Phytochemical Analysis of Ficus religiosa

The use of F. religiosa might be beneficial in inflammatory illnesses and can be used for a variety of health conditions. In this article, we studied the identification of antioxidants using (DPPH) 2,2-Diphenyl-1-picrylhydrazylradical scavenging activity in Ficus religiosa, as F. religiosa is an important herbal plant, and every part of it has various medicinal properties such as antibacterial properties that can be used by the researchers in the development and design of various new drugs. The 2,2-Diphenyl-1-picrylhydrazyl (DPPH) is a popular, quick, easy, and affordable approach for the measurement of antioxidant properties that includes the use of the free radicals used for assessing the potential of substances to serve as hydrogen providers or free-radical scavengers (FRS). The technique of DPPH testing is associated with the elimination of DPPH, which would be a stabilized free radical. The free-radical DPPH interacts with an odd electron to yield a strong absorbance at 517 nm, i.e., a purple hue. An FRS antioxidant, for example, reacts to DPPH to form DPPHH, which has a lower absorbance than DPPH because of the lower amount of hydrogen. It is radical in comparison to the DPPH-H form, because it causes decolorization, or a yellow hue, as the number of electrons absorbed increases. Decolorization affects the lowering capacity significantly. As soon as the DPPH solutions are combined with the hydrogen atom source, the lower state of diphenylpicrylhydrazine is formed, shedding its violet color. To explain the processes behind the DPPH tests, as well as their applicability to Ficus religiosa (F. religiosa) in the manufacture of metal oxide nanoparticles, in particular MgO, and their influence on antioxidants, a specimen from the test was chosen for further study. According to our findings, F. religiosa has antioxidant qualities and may be useful in the treatment of disorders caused by free radicals.

更新日期：2022-02-17

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