Ultra-centrifugation force in adaptive evolution changes the cell structure of oleaginous yeast Trichosporon cutaneum into a favorable space for lipid accumulation,Biotechnology and Bioengineering

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Ultra-centrifugation force in adaptive evolution changes the cell structure of oleaginous yeast Trichosporon cutaneum into a favorable space for lipid accumulation
Biotechnology and Bioengineering ( IF 3.5 ) Pub Date : 2022-02-14 , DOI: 10.1002/bit.28060
Qi Liu ₁ , Minping Lu ₁ , Ci Jin ₁ , Weiliang Hou ₁ , Liao Zhao ₁ , Jie Bao ₁

Affiliation

Microbial lipid production from lignocellulose biomass provides an essential option for sustainable and carbon-neutral supply of future aviation fuels, biodiesel, as well as various food and nutrition products. Oleaginous yeast is the major microbial cell factory but its lipid-producing performance is far below the requirements of industrial application. Here we show an ultra-centrifugation fractionation in adaptive evolution (UCF) of Trichosporon cutaneum based on the minor cell density difference. The lightest cells with the maximum intracellular lipid content were isolated by ultra-centrifugation fractionation in the long-term adaptive evolution. Significant changes occurred in the cell morphology with a fragile cell wall wrapping and enlarged intracellular space (two orders of magnitude increase in cell size). Complete and coordinate assimilations of all nonglucose sugars derived from lignocellulose were triggered and fluxed into lipid synthesis. Genome mutations and significant transcriptional regulations of the genes responsible for cell structure were identified and experimentally confirmed. The obtained T. cutaneum MP11 cells achieved a high lipid production of wheat straw, approximately five-fold greater than that of the parental cells. The study provided an effective method for screening the high lipid-containing oleaginous yeast cells as well as the intracellular products accumulating cells in general.

中文翻译：

适应性进化中的超离心力将产油酵母皮肤毛孢菌的细胞结构改变为脂质积累的有利空间

从木质纤维素生物质生产微生物脂质为未来航空燃料、生物柴油以及各种食品和营养产品的可持续和碳中和供应提供了重要选择。产油酵母是主要的微生物细胞工厂，但其产脂性能远低于工业应用的要求。在这里，我们展示了皮肤毛孢菌适应性进化 (UCF) 中的超离心分级分离基于微小的细胞密度差异。在长期适应性进化中，通过超离心分离分离出具有最大细胞内脂质含量的最轻细胞。细胞形态发生显着变化，细胞壁包裹脆弱，细胞内空间扩大（细胞大小增加两个数量级）。源自木质纤维素的所有非葡萄糖的完全和协调同化被触发并流入脂质合成中。鉴定和实验证实了负责细胞结构的基因的基因组突变和重要的转录调控。获得的T. cutaneumMP11 细胞实现了小麦秸秆的高脂质产量，大约是亲本细胞的五倍。该研究为筛选高脂含油酵母细胞以及一般的胞内产物蓄积细胞提供了一种有效的方法。

更新日期：2022-02-14

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