Background: Acid Blue 113 (AB113) is a typical azo dye, and the resulting wastewater is toxic and difficult to remove. Methods: The experimental culture was set up for the biodegradation of the azo dye AB113, and the cell growth and dye decolorization were monitored. Transcriptome sequencing was performed in the presence and absence of AB113 treatment. The key pathways and enzymes involved in AB113 degradation were found through pathway analysis and enrichment software (GO, EggNog and KEGG). Results: S. melonis B-2 achieved more than 80% decolorization within 24 h (50 and 100 mg/L dye). There was a positive relationship between cell growth and the azo dye degradation rate. The expression level of enzymes involved in benzoate and naphthalene degradation pathways (NADH quinone oxidoreductase, N-acetyltransferase and aromatic ring-hydroxylating dioxygenase) increased significantly after the treatment of AB113. Conclusions: Benzoate and naphthalene degradation pathways were the key pathways for AB113 degradation. NADH quinone oxidoreductase, N-acetyltransferase, aromatic ring-hydroxylating dioxygenase and CYP450 were the key enzymes for AB113 degradation. This study provides evidence for the process of AB113 biodegradation at the molecular and biochemical level that will be useful in monitoring the dye wastewater treatment process at the full-scale treatment.

中文翻译：

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染料废水处理过程中甜瓜鞘氨醇单胞菌 B-2 中偶氮染料酸性蓝 113 降解途径的转录组学分析

背景：酸性蓝 113 (AB113) 是一种典型的偶氮染料，产生的废水有毒且难以去除。方法：建立偶氮染料AB113生物降解实验培养物，监测细胞生长和染料脱色情况。在存在和不存在 AB113 处理的情况下进行转录组测序。通过途径分析和富集软件（GO、EggNog和KEGG）发现了参与AB113降解的关键途径和酶。结果：S.melonis B-2 在 24 小时内实现了 80% 以上的脱色（50 和 100 mg/L 染料）。细胞生长与偶氮染料降解率呈正相关。涉及苯甲酸盐和萘降解途径的酶（NADH 醌氧化还原酶，N-乙酰转移酶和芳香环羟基化双加氧酶）在 AB113 处理后显着增加。结论：苯甲酸酯和萘降解途径是AB113降解的关键途径。NADH醌氧化还原酶、N-乙酰转移酶、芳环羟基化双加氧酶和CYP450是AB113降解的关键酶。该研究为 AB113 在分子和生化水平上的生物降解过程提供了证据，这将有助于在全面处理中监测染料废水处理过程。芳香环羟基化双加氧酶和CYP450是AB113降解的关键酶。该研究为 AB113 在分子和生化水平上的生物降解过程提供了证据，这将有助于在全面处理中监测染料废水处理过程。芳香环羟基化双加氧酶和CYP450是AB113降解的关键酶。该研究为 AB113 在分子和生化水平上的生物降解过程提供了证据，这将有助于在全面处理中监测染料废水处理过程。