Abstract

Context

Delphinidin-3-O-glucoside (DP) is a bioactive compound of Hibiscus sabdariffa L. (Malvaceae) (Roselle) calyces and exerts endothelial protection and lipid-lowering activities, which provided a basis for the prevention and treatment of cardiovascular diseases.

Objectives

To investigate the therapeutic effects of DP against atherosclerosis.

Materials and methods

A rabbit model of atherosclerosis (AS) was established by 12 weeks of a high-fat diet (HFD). The rabbits were divided into five groups: control, AS, simvastatin (4 mg/kg), and two DP groups (10 and 20 mg/kg). After treatment with DP or simvastatin by oral gavage for 12 weeks, the lipid profiles were measured. Histopathological assessment of the aorta was performed by H&E staining. Oxidative stress and inflammation-related markers were analyzed by ELISA kit and real-time RT-PCR.

Results

DP (20 mg/kg) decreased serum TG (2.36 ± 0.66 vs. 4.33 ± 0.27 mmol/L for the AS group), TC, LDL-C, and HDL-C (all p < 0.05). DP (20 mg/kg) also reduced lipid levels in the liver and aorta. DP (20 mg/kg) down-regulated the mRNA levels of IL-6, VCAM-1, and NF-κB and up-regulated the mRNA levels of GSH-PX and SOD1.

Conclusions

This study proved that DP alleviated the HFD-induced oxidative stress and inflammation in atherosclerosis rabbits. These results provided the scientific basis for developing novel therapies.

中文翻译：

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Delphinidin-3-O-glucoside 是 Hibiscus sabdariffa calyces 的一种活性化合物，可抑制兔动脉粥样硬化的氧化应激和炎症

摘要

语境

Delphinidin-3- O - glucoside (DP)是锦葵科(Malvaceae)(Roselle)花萼的一种生物活性化合物，具有保护内皮和降脂的作用，为心血管疾病的防治提供了基础。

目标

探讨DP对动脉粥样硬化的治疗作用。

材料和方法

通过12周的高脂饮食（HFD）建立兔动脉粥样硬化（AS）模型。将兔子分为五组：对照组、AS、辛伐他汀（4 mg/kg）和两个DP组（10和20 mg/kg）。通过口服强饲法用 DP 或辛伐他汀治疗 12 周后，测量血脂谱。通过H&E染色进行主动脉的组织病理学评估。通过ELISA试剂盒和实时RT-PCR分析氧化应激和炎症相关标志物。

结果

DP (20 mg/kg) 降低血清 TG（AS 组为 2.36 ± 0.66 vs. 4.33 ± 0.27 mmol/L）、TC、LDL-C 和 HDL-C（所有p  < 0.05）。DP (20 mg/kg) 还降低了肝脏和主动脉中的脂质水平。DP (20 mg/kg) 下调 IL-6、VCAM-1 和 NF-κB 的 mRNA 水平，并上调 GSH-PX 和 SOD1 的 mRNA 水平。

结论

本研究证明DP减轻了HFD诱导的动脉粥样硬化兔的氧化应激和炎症。这些结果为开发新疗法提供了科学依据。