Cryo-EM of Helical Polymers,Chemical Reviews

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Cryo-EM of Helical Polymers
Chemical Reviews ( IF 51.4 ) Pub Date : 2022-02-08 , DOI: 10.1021/acs.chemrev.1c00753
Fengbin Wang ₁ , Ordy Gnewou ₂ , Armin Solemanifar _{2,

3} , Vincent P Conticello ₂ , Edward H Egelman ₁

Affiliation

While the application of cryogenic electron microscopy (cryo-EM) to helical polymers in biology has a long history, due to the huge number of helical macromolecular assemblies in viruses, bacteria, archaea, and eukaryotes, the use of cryo-EM to study synthetic soft matter noncovalent polymers has been much more limited. This has mainly been due to the lack of familiarity with cryo-EM in the materials science and chemistry communities, in contrast to the fact that cryo-EM was developed as a biological technique. Nevertheless, the relatively few structures of self-assembled peptide nanotubes and ribbons solved at near-atomic resolution by cryo-EM have demonstrated that cryo-EM should be the method of choice for a structural analysis of synthetic helical filaments. In addition, cryo-EM has also demonstrated that the self-assembly of soft matter polymers has enormous potential for polymorphism, something that may be obscured by techniques such as scattering and spectroscopy. These cryo-EM structures have revealed how far we currently are from being able to predict the structure of these polymers due to their chaotic self-assembly behavior.

中文翻译：

螺旋聚合物的冷冻电镜

虽然低温电子显微镜（cryo-EM）在生物学中对螺旋聚合物的应用有着悠久的历史，但由于病毒、细菌、古细菌和真核生物中存在大量的螺旋大分子组装体，使用冷冻电子显微镜来研究合成聚合物软物质非共价聚合物受到的限制要大得多。这主要是由于材料科学和化学界对冷冻电镜缺乏了解，而冷冻电镜是作为一种生物技术而开发的。然而，通过冷冻电镜以近原子分辨率解析的自组装肽纳米管和带的结构相对较少，这表明冷冻电镜应该成为合成螺旋丝结构分析的首选方法。此外，冷冻电镜还证明，软物质聚合物的自组装具有多晶型的巨大潜力，而散射和光谱等技术可能会掩盖这一点。这些冷冻电镜结构揭示了我们目前距离预测这些聚合物的结构还有多远，因为它们具有混沌的自组装行为。

更新日期：2022-02-08

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